Basic Fibroblast Growth Factor Induces Osteoclast Formation in Murine Bone Marrow Cultures

We determined the effect of basic fibroblast growth factor (bFGF) on osteoclast-like cell (OCL) formation in bone marrow cultures using C57BL/6 mice. Cells were cultured for 7 days with or without bFGF at various concentrations or 10 −8 mol/L 1,25(OH) 2 vitamin D 3 [1,25(OH) 2D 3]. bFGF dose-depende...

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Veröffentlicht in:	Bone (New York, N.Y.) N.Y.), 1998-04, Vol.22 (4), p.309-316
Hauptverfasser:	Hurley, M.M, Lee, S.K, Raisz, L.G, Bernecker, P, Lorenzo, J
Format:	Artikel
Sprache:	eng
Schlagworte:	1,25-Dihydroxyvitamin D 3 Actins - biosynthesis Animals Basic fibroblast growth factor Biological and medical sciences Bone Marrow Cells - cytology Bone Marrow Cells - drug effects Calcitonin receptor Calcitriol - pharmacology Cell Differentiation - drug effects Cell Division - drug effects Cells, Cultured Dinoprostone - biosynthesis Dinoprostone - pharmacology Femur - cytology Fibroblast Growth Factor 2 - pharmacology Fundamental and applied biological sciences. Psychology Humerus - cytology Mice Mice, Inbred C57BL Osteoclastogenesis Osteoclasts - cytology Osteoclasts - drug effects Prostaglandin Receptors, Calcitonin - biosynthesis Receptors, Calcitonin - genetics Resorption pit RNA, Messenger - analysis Skeleton and joints Space life sciences Tibia - cytology Time Factors Vertebrates: osteoarticular system, musculoskeletal system
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Zusammenfassung:	We determined the effect of basic fibroblast growth factor (bFGF) on osteoclast-like cell (OCL) formation in bone marrow cultures using C57BL/6 mice. Cells were cultured for 7 days with or without bFGF at various concentrations or 10 −8 mol/L 1,25(OH) 2 vitamin D 3 [1,25(OH) 2D 3]. bFGF dose-dependently increased OCL formation per well (10 −10 mol/L = 40 ± 2; 10 −9 mol/L = 146 ± 13; 10 −8 mol/L = 156 ± 12) compared with control (
ISSN:	8756-3282 1873-2763
DOI:	10.1016/S8756-3282(97)00292-5