Relationship between lucigenin-enhanced chemiluminescence and uroporphyrinogen oxidation in mouse and chick embryo liver microsomes

Three approaches have been used to detect reactive oxygen species production in such cytochrome P450-mediated reactions: lipid peroxidation, chemiluminescence and uroporphyrinogen oxidation. We have now used liver microsomes from chick embryos and mice treated with known inducers of cytochrome P450...

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Veröffentlicht in:	Biochemical pharmacology 1990-06, Vol.39 (11), p.1828-1830
Hauptverfasser:	Sinclair, Peter R., Jacobs, Judith M., Deam, Susan M., Lambrecht, Richard W., Sinclair, Jacqueline F., Elder, George H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acridines - pharmacology Animals Biological and medical sciences Chemical and industrial products toxicology. Toxic occupational diseases chemiluminescence Chick Embryo Cytochrome P-450 Enzyme System - metabolism lucigenin Luminescent Measurements Male Medical sciences Mice Mice, Inbred AKR Mice, Inbred C57BL Mice, Inbred DBA microsomes Microsomes, Liver - metabolism Oxidation-Reduction Porphyrinogens - metabolism Toxicology uroporphyrinogen Uroporphyrinogens - metabolism Various organic compounds
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Zusammenfassung:	Three approaches have been used to detect reactive oxygen species production in such cytochrome P450-mediated reactions: lipid peroxidation, chemiluminescence and uroporphyrinogen oxidation. We have now used liver microsomes from chick embryos and mice treated with known inducers of cytochrome P450 and compared the relative rates of CL and uroporphyrinogen oxidation. From the data obtained, we conclude that these two reactions do not always measure the same phenomenon.
ISSN:	0006-2952 1873-2968
DOI:	10.1016/0006-2952(90)90135-8