A two-dimensional gel protein database of noncultured total normal human epidermal keratinocytes: Identification of proteins strongly up-regulated in psoriatic epidermis

A two‐dimensional (2‐D) gel database of proteins from noncultured total normal human epidermal keratinocytes has been established. A total of 1449 [35S]methionine labelled proteins (1112 isoelectric focusing, 337 nonequilibrium pH gradient electrophoresis) were resolved and recorded using computer assisted (PDQ‐SCAN and PDQUEST software) 2‐D gel electrophoresis. By matching the protein patterns of total keratinocytes and transformed human amnion cells (master database; Celis et al., Leukemia 1988, 2, 561–602) as well as by 2‐D immunoblotting and microsequencing of keratinocyte proteins, it was possible to identify 72 polypeptides in the keratinocyte database. The database also includes data on polypeptides that are synthesized at a higher level by keratinocytes enriched in basal cells, and on six secreted proteins which are produced, albeit at a reduced rate, by normal keratinocytes and that are strongly up‐regulated in psoriatic epidermis (Celis et al., FEBS Letters, in press).