An evaluation of the hypo-osmotic sperm swelling test as a predictor of fertilizing capacity in vitro

Summary The ability of sperm to swell in hypo‐osmotic conditions was examined in 211 semen samples from the partners of patients about to undergo oocyte retrieval for in‐vitro fertilization (IVF). The test was performed using aliquots of semen, the remainder of which was then prepared for IVF. No significant difference was found, in either the percentage of swollen sperm or the type of swelling response, between samples that achieved fertilization in vitro and those that did not, or between any of the diagnostic categories of infertility (tubal damage, unexplained infertility, oligospermia). In samples which achieved fertilization in vitro there were correlations between sperm swelling and sperm motility (r = ‐0.51) and abnormal morphology (r = 0.33), but no such correlations were demonstrated in samples that failed to achieve fertilization. Moreover, there was no significant difference between the percentage of swollen sperm in semen (mean motility 64%), in samples immediately after preparation for IVF (mean motility 96%) or in capacitated sperm 24 h after preparation (mean motility 91%). These results demonstrate that the hypo‐osmotic sperm swelling test does not assist in the prediction of the fertilizing capacity of human sperm in vitro.