Affinity of fibroblast growth factors for β-cyclodextrin tetradecasulfate

β-Cyclodextrin tetradecasulfate was found to have a very strong affinity for fibroblast growth factor (FGF) and could substitute for heparin in FGF purification. Basic FGF was purified about 200,000-fold from a rat chondrosarcoma using a method of biaffinity chromatography in which the β-cyclodextri...

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Veröffentlicht in:	Analytical biochemistry 1990-02, Vol.185 (1), p.108-111
Hauptverfasser:	Shing, Yuen, Folkman, Judah, Weisz, Paul B., Joullie, Madeleine M., Ewing, William R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical biochemistry: general aspects, technics, instrumentation Analytical, structural and metabolic biochemistry Animals beta-Cyclodextrins Biological and medical sciences Cells, Cultured Chromatography, Affinity - methods Cyclodextrins Dextrins Electrophoresis, Polyacrylamide Gel Fibroblast Growth Factors - isolation & purification Fundamental and applied biological sciences. Psychology Humans Mice Mice, Inbred BALB C Protein Binding Rats Recombinant Proteins Starch
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Zusammenfassung:	β-Cyclodextrin tetradecasulfate was found to have a very strong affinity for fibroblast growth factor (FGF) and could substitute for heparin in FGF purification. Basic FGF was purified about 200,000-fold from a rat chondrosarcoma using a method of biaffinity chromatography in which the β-cyclodextrin tetradecasulfate polymer was mixed with copper-Sepharose. This method takes advantage of the strong affinity of FGF for both β-cyclodextrin tetradecasulfate and copper.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(90)90263-9