Serine Protease of Hepatitis C Virus Expressed in Insect Cells as the NS3/4A Complex

Hepatitis C virus (HCV) protease NS3 and its protein activator NS4A participate in the processing of the viral polyprotein into its constituent nonstructural proteins. The NS3/4A complex is thus an attractive target for antiviral therapy against HCV. We expressed the full-length NS3 and NS4A in inse...

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Veröffentlicht in:	Biochemistry (Easton) 1998-03, Vol.37 (10), p.3392-3401
Hauptverfasser:	Sali, Dasa Lipovsek, Ingram, Richard, Wendel, Michele, Gupta, Divya, McNemar, Charles, Tsarbopoulos, Anthony, Chen, Janice W, Hong, Zhi, Chase, Robert, Risano, Christine, Zhang, Rumin, Yao, Nanhua, Kwong, Ann D, Ramanathan, Lata, Le, Hung V, Weber, Patricia C
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphatases - metabolism Amino Acid Sequence Animals Baculoviridae - genetics Cell Line Cloning, Molecular DNA Helicases - metabolism Gene Expression Genes, Viral Hepacivirus - enzymology Hepacivirus - genetics Kinetics Molecular Sequence Data Protein Structure, Secondary Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - isolation & purification Recombinant Fusion Proteins - metabolism Serine Endopeptidases - genetics Serine Endopeptidases - isolation & purification Serine Endopeptidases - metabolism Spodoptera Viral Nonstructural Proteins - genetics Viral Nonstructural Proteins - isolation & purification Viral Nonstructural Proteins - metabolism
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