Decreased binding of calmodulin to bull sperm proteins during heparin-induced capacitation
The 125I-calmodulin gel overlay procedure was used to evaluate the effect of a heparin treatment on the calmodulin-binding proteins of bull spermatozoa. At concentrations that increase the in vitro fertilization rate of in vitro-matured oocytes, heparin induced a decrease in the binding to calmoduli...
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Veröffentlicht in: | Biology of reproduction 1990-03, Vol.42 (3), p.483-489 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The 125I-calmodulin gel overlay procedure was used to evaluate the effect of a heparin treatment on the calmodulin-binding
proteins of bull spermatozoa. At concentrations that increase the in vitro fertilization rate of in vitro-matured oocytes,
heparin induced a decrease in the binding to calmodulin (CaM) in 3 sperm proteins of 28, 30, and 49 kDa. The binding of these
proteins to CaM was higher when Ca2+ was absent from the overlay procedure, and this binding was negatively correlated to
the fertilization rate. These results suggest that sperm capacitation is associated with a decrease in the binding of CaM
to the 28, 30, and 49 kDa sperm CaM-binding proteins. Implications of such a decrease are discussed. |
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ISSN: | 0006-3363 1529-7268 |
DOI: | 10.1095/biolreprod42.3.483 |