Decreased binding of calmodulin to bull sperm proteins during heparin-induced capacitation

The 125I-calmodulin gel overlay procedure was used to evaluate the effect of a heparin treatment on the calmodulin-binding proteins of bull spermatozoa. At concentrations that increase the in vitro fertilization rate of in vitro-matured oocytes, heparin induced a decrease in the binding to calmoduli...

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Veröffentlicht in:Biology of reproduction 1990-03, Vol.42 (3), p.483-489
Hauptverfasser: Leclerc, P. (Centre de Researches du CHUL Ste-Foy, Quebec, Canada), Sirard, M.A, Chafouleas, J.G, Lambert, R.D
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Sprache:eng
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Zusammenfassung:The 125I-calmodulin gel overlay procedure was used to evaluate the effect of a heparin treatment on the calmodulin-binding proteins of bull spermatozoa. At concentrations that increase the in vitro fertilization rate of in vitro-matured oocytes, heparin induced a decrease in the binding to calmodulin (CaM) in 3 sperm proteins of 28, 30, and 49 kDa. The binding of these proteins to CaM was higher when Ca2+ was absent from the overlay procedure, and this binding was negatively correlated to the fertilization rate. These results suggest that sperm capacitation is associated with a decrease in the binding of CaM to the 28, 30, and 49 kDa sperm CaM-binding proteins. Implications of such a decrease are discussed.
ISSN:0006-3363
1529-7268
DOI:10.1095/biolreprod42.3.483