Chemotherapy screening assay using 3-dimensional cell culture

A new alginate culture method (ACM) was used to test the effects of vincristine and 5-fluorouracil (5-FU) on the commonly used human colon carcinoma cell line HT-29. Colorimetric analysis of viability following treatment with physiologically tolerated levels of vincristine showed significant reducti...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Cancer letters 1990-05, Vol.51 (1), p.11-16
Hauptverfasser:	Kupchik, H.Z., Collins, E.A., O'Brien, M.J., McCaffrey, R.P.
Format:	Artikel
Sprache:	eng
Schlagworte:	3-D culture alginate Alginates Antineoplastic agents Biological and medical sciences Cell Survival - drug effects chemosensitivity Clone Cells - drug effects colon carcinoma Colonic Neoplasms Drug Screening Assays, Antitumor - methods Fluorouracil - pharmacology General aspects Humans Medical sciences organoid growth Pharmacology. Drug treatments Tumor Cells, Cultured - drug effects Vincristine - pharmacology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A new alginate culture method (ACM) was used to test the effects of vincristine and 5-fluorouracil (5-FU) on the commonly used human colon carcinoma cell line HT-29. Colorimetric analysis of viability following treatment with physiologically tolerated levels of vincristine showed significant reduction of the surviving cell population. Hematoxylin and eosin staining of sections of treated organoid growths corroborated the colorimetric analyses. The ACM is inexpensive, rapid and extremely versatile. Many alginate beads containing “mini-tissue” growths of many cell lines can be used simultaneously to evaluate numerous chemotherapeutic agents in an “in vivo-like” environment.
ISSN:	0304-3835 1872-7980
DOI:	10.1016/0304-3835(90)90224-L