Recommended storage and resuscitation conditions for the MDCC-MSB1 cell line
Storage and resuscitation of MDCC-MSB1 cells, most commonly used for the propagation of chicken anemia virus was studied. Cells were frozen slowly in RPMI medium 1640 with 10% or 20% fetal bovine serum and 20% dimethyl sulfoxide, held at -70 C overnight, and placed into liquid nitrogen the next day....
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| Veröffentlicht in: | Avian diseases 1998-01, Vol.42 (1), p.168-172 |
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| container_title | Avian diseases |
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| creator | Coombes, A.L. (Charles Stuart University, Wagga Wagga, NSW, Australia.) Crawford, G.R |
| description | Storage and resuscitation of MDCC-MSB1 cells, most commonly used for the propagation of chicken anemia virus was studied. Cells were frozen slowly in RPMI medium 1640 with 10% or 20% fetal bovine serum and 20% dimethyl sulfoxide, held at -70 C overnight, and placed into liquid nitrogen the next day. After 1 mo, 91% to 100% resuscitation rates were obtained, while cells that were frozen fast (directly transferred into liquid nitrogen) could not be resuscitated |
| doi_str_mv | 10.2307/1592590 |
| format | Article |
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(Charles Stuart University, Wagga Wagga, NSW, Australia.)</creatorcontrib><creatorcontrib>Crawford, G.R</creatorcontrib><title>Recommended storage and resuscitation conditions for the MDCC-MSB1 cell line</title><title>Avian diseases</title><addtitle>Avian Dis</addtitle><description>Storage and resuscitation of MDCC-MSB1 cells, most commonly used for the propagation of chicken anemia virus was studied. Cells were frozen slowly in RPMI medium 1640 with 10% or 20% fetal bovine serum and 20% dimethyl sulfoxide, held at -70 C overnight, and placed into liquid nitrogen the next day. After 1 mo, 91% to 100% resuscitation rates were obtained, while cells that were frozen fast (directly transferred into liquid nitrogen) could not be resuscitated</description><subject>ALBUMINAS</subject><subject>ALBUMINE</subject><subject>ALBUMINS</subject><subject>ALMACENAMIENTO</subject><subject>Animals</subject><subject>BIOCHEMISTRY</subject><subject>BIOCHIMIE</subject><subject>BIOQUIMICA</subject><subject>Blood</subject><subject>BOVINE SERUM ALBUMIN</subject><subject>Cattle</subject><subject>CELL CULTURE</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell growth</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Cell membranes</subject><subject>Cell Survival</subject><subject>CHICKEN ANEMIA VIRUS</subject><subject>Chicken anemia virus - growth & development</subject><subject>Chickens</subject><subject>CIRCOVIRIDAE</subject><subject>COMPOSE ORGANOSOUFRE</subject><subject>COMPUESTO ORGANICO DEL AZUFRE</subject><subject>Cryopreservation - methods</subject><subject>CULTIVO DE CELULAS</subject><subject>CULTURE DE CELLULE</subject><subject>Culture Media</subject><subject>DECONGELATION</subject><subject>DESCONGELACION</subject><subject>DIMETHYL SULFOXIDE</subject><subject>Freezing</subject><subject>Ice</subject><subject>Liquid nitrogen</subject><subject>METHODE</subject><subject>METHODS</subject><subject>METODOS</subject><subject>ORGANOSULPHUR COMPOUNDS</subject><subject>Research Notes</subject><subject>Resuscitation</subject><subject>STOCKAGE</subject><subject>STORAGE</subject><subject>Storage conditions</subject><subject>THAWING</subject><issn>0005-2086</issn><issn>1938-4351</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1PwzAMhiMEGmMgfgFSDghOhaRpmuYI5VPahMTYuUoTZ3Ram5G0B_49rVZx3cmW_ei19SB0ScldzIi4p1zGXJIjNKWSZVHCOD1GU0IIj2KSpafoLIQNIVTIlEzQRHLGiORTNP8E7eoaGgMGh9Z5tQasGoM9hC7oqlVt5RqsXWOqoQvYOo_bb8CLpzyPFstHijVst3hbNXCOTqzaBrgY6wytXp6_8rdo_vH6nj_MI8141kYWNMScKyWSLEttUiYJs1oAMdL0A6VjSGnKy4RbYZKUcakAmIEys7EWQrEZutnn7rz76SC0RV2F4QvVgOtCIaQQVPL0INhfiRPJWA_e7kHtXQgebLHzVa38b0FJMQguRsE9eTVGdmUN5p8bjfb76_1-M9g8HGOVK9TaV6FYLamUgsiYyZT9ATfaiFQ</recordid><startdate>19980101</startdate><enddate>19980101</enddate><creator>Coombes, A.L. 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(Charles Stuart University, Wagga Wagga, NSW, Australia.)</au><au>Crawford, G.R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Recommended storage and resuscitation conditions for the MDCC-MSB1 cell line</atitle><jtitle>Avian diseases</jtitle><addtitle>Avian Dis</addtitle><date>1998-01-01</date><risdate>1998</risdate><volume>42</volume><issue>1</issue><spage>168</spage><epage>172</epage><pages>168-172</pages><issn>0005-2086</issn><eissn>1938-4351</eissn><abstract>Storage and resuscitation of MDCC-MSB1 cells, most commonly used for the propagation of chicken anemia virus was studied. Cells were frozen slowly in RPMI medium 1640 with 10% or 20% fetal bovine serum and 20% dimethyl sulfoxide, held at -70 C overnight, and placed into liquid nitrogen the next day. After 1 mo, 91% to 100% resuscitation rates were obtained, while cells that were frozen fast (directly transferred into liquid nitrogen) could not be resuscitated</abstract><cop>United States</cop><pub>American Association of Avian Pathologists, Inc</pub><pmid>9533095</pmid><doi>10.2307/1592590</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0005-2086 |
| ispartof | Avian diseases, 1998-01, Vol.42 (1), p.168-172 |
| issn | 0005-2086 1938-4351 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79771956 |
| source | Jstor Complete Legacy; MEDLINE |
| subjects | ALBUMINAS ALBUMINE ALBUMINS ALMACENAMIENTO Animals BIOCHEMISTRY BIOCHIMIE BIOQUIMICA Blood BOVINE SERUM ALBUMIN Cattle CELL CULTURE Cell Culture Techniques - methods Cell growth Cell Line Cell lines Cell membranes Cell Survival CHICKEN ANEMIA VIRUS Chicken anemia virus - growth & development Chickens CIRCOVIRIDAE COMPOSE ORGANOSOUFRE COMPUESTO ORGANICO DEL AZUFRE Cryopreservation - methods CULTIVO DE CELULAS CULTURE DE CELLULE Culture Media DECONGELATION DESCONGELACION DIMETHYL SULFOXIDE Freezing Ice Liquid nitrogen METHODE METHODS METODOS ORGANOSULPHUR COMPOUNDS Research Notes Resuscitation STOCKAGE STORAGE Storage conditions THAWING |
| title | Recommended storage and resuscitation conditions for the MDCC-MSB1 cell line |
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