Recommended storage and resuscitation conditions for the MDCC-MSB1 cell line

Storage and resuscitation of MDCC-MSB1 cells, most commonly used for the propagation of chicken anemia virus was studied. Cells were frozen slowly in RPMI medium 1640 with 10% or 20% fetal bovine serum and 20% dimethyl sulfoxide, held at -70 C overnight, and placed into liquid nitrogen the next day....

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Veröffentlicht in:	Avian diseases 1998-01, Vol.42 (1), p.168-172
Hauptverfasser:	Coombes, A.L. (Charles Stuart University, Wagga Wagga, NSW, Australia.), Crawford, G.R
Format:	Artikel
Sprache:	eng
Schlagworte:	ALBUMINAS ALBUMINE ALBUMINS ALMACENAMIENTO Animals BIOCHEMISTRY BIOCHIMIE BIOQUIMICA Blood BOVINE SERUM ALBUMIN Cattle CELL CULTURE Cell Culture Techniques - methods Cell growth Cell Line Cell lines Cell membranes Cell Survival CHICKEN ANEMIA VIRUS Chicken anemia virus - growth & development Chickens CIRCOVIRIDAE COMPOSE ORGANOSOUFRE COMPUESTO ORGANICO DEL AZUFRE Cryopreservation - methods CULTIVO DE CELULAS CULTURE DE CELLULE Culture Media DECONGELATION DESCONGELACION DIMETHYL SULFOXIDE Freezing Ice Liquid nitrogen METHODE METHODS METODOS ORGANOSULPHUR COMPOUNDS Research Notes Resuscitation STOCKAGE STORAGE Storage conditions THAWING
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Beschreibung
Zusammenfassung:	Storage and resuscitation of MDCC-MSB1 cells, most commonly used for the propagation of chicken anemia virus was studied. Cells were frozen slowly in RPMI medium 1640 with 10% or 20% fetal bovine serum and 20% dimethyl sulfoxide, held at -70 C overnight, and placed into liquid nitrogen the next day. After 1 mo, 91% to 100% resuscitation rates were obtained, while cells that were frozen fast (directly transferred into liquid nitrogen) could not be resuscitated
ISSN:	0005-2086 1938-4351
DOI:	10.2307/1592590