Arachidonic acid inhibits both K+ and Ca2+ currents in isolated type I cells of the rat carotid body
Whole-cell patch-clamp recordings were used to investigate the effects of arachidonic acid (AA) on K+ and Ca2+ channels in isolated rat type I carotid body cells. AA (2-20 microM) produced a concentration-dependent inhibition of both K+ currents and Ca2+ channel currents. The effects of AA on K+ cur...
Gespeichert in:
| Veröffentlicht in: | Brain research 1998-03, Vol.787 (2), p.315-320 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 320 |
|---|---|
| container_issue | 2 |
| container_start_page | 315 |
| container_title | Brain research |
| container_volume | 787 |
| creator | HATTON, C. J PEERS, C |
| description | Whole-cell patch-clamp recordings were used to investigate the effects of arachidonic acid (AA) on K+ and Ca2+ channels in isolated rat type I carotid body cells. AA (2-20 microM) produced a concentration-dependent inhibition of both K+ currents and Ca2+ channel currents. The effects of AA on K+ currents were unaffected by indomethacin (5 microM), phenidone (5 microM) or 1-aminobenzotriazole (3 mM), suggesting that AA did not exert its effects via cyclo-oxygenase, lipoxygenase or cytochrome P-450 (cP-450) metabolism. Our results suggest that AA directly and non-selectively inhibits ionic currents in rat type I carotid body cells. |
| doi_str_mv | 10.1016/S0006-8993(97)01486-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79763086</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>79763086</sourcerecordid><originalsourceid>FETCH-LOGICAL-c265t-2664b7c002eb8d8bac82ce7015777e1ca9f95cf505efb7d3559c42901c6a04a23</originalsourceid><addsrcrecordid>eNo9kM1LJDEQxcOi6KzunyDkILKL9JqkO19HGfZDVvCweg7VlTQT6emMSeYw_709OsypeLz3qoofIVec_eSMq7v_jDHVGGvb71b_YLwzs_pCFtxo0SjRsROyOEbOyddSXmfZtpadkTMruVFKL4i_z4Cr6NMUkQJGT-O0in2shfaprui_WwqTp0sQtxS3OYdpduJEY0kj1OBp3W0CfaAYxrHQNNC6CjRDpQg51Xldn_zukpwOMJbw7TAvyMvvX8_Lv83j05-H5f1jg0LJ2gilul4jYyL0xpse0AgMmnGptQ4cwQ5W4iCZDEOvfSulxU5YxlEB60C0F-Tmc-8mp7dtKNWtY9l_BlNI2-K01aplRs1B-RnEnErJYXCbHNeQd44zt6frPui6PTpntfug68zcuzoc2Pbr4I-tA87Zvz74UBDGIcOEsRxjQvDWatO-A6hAgTM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>79763086</pqid></control><display><type>article</type><title>Arachidonic acid inhibits both K+ and Ca2+ currents in isolated type I cells of the rat carotid body</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>HATTON, C. J ; PEERS, C</creator><creatorcontrib>HATTON, C. J ; PEERS, C</creatorcontrib><description>Whole-cell patch-clamp recordings were used to investigate the effects of arachidonic acid (AA) on K+ and Ca2+ channels in isolated rat type I carotid body cells. AA (2-20 microM) produced a concentration-dependent inhibition of both K+ currents and Ca2+ channel currents. The effects of AA on K+ currents were unaffected by indomethacin (5 microM), phenidone (5 microM) or 1-aminobenzotriazole (3 mM), suggesting that AA did not exert its effects via cyclo-oxygenase, lipoxygenase or cytochrome P-450 (cP-450) metabolism. Our results suggest that AA directly and non-selectively inhibits ionic currents in rat type I carotid body cells.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/S0006-8993(97)01486-8</identifier><identifier>PMID: 9518667</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier</publisher><subject>Animals ; Arachidonic Acid - pharmacology ; Biological and medical sciences ; Calcium Channel Blockers - pharmacology ; Calcium Channels - metabolism ; Cardiorespiratory control. Arterial mecano- and chemoreceptor ; Carotid Body - drug effects ; Carotid Body - enzymology ; Carotid Body - metabolism ; Cyclooxygenase Inhibitors - pharmacology ; Cytochrome P-450 Enzyme Inhibitors ; Electrophysiology ; Fundamental and applied biological sciences. Psychology ; Lipoxygenase Inhibitors - pharmacology ; Patch-Clamp Techniques ; Potassium Channel Blockers ; Rats ; Rats, Wistar ; Vertebrates: respiratory system</subject><ispartof>Brain research, 1998-03, Vol.787 (2), p.315-320</ispartof><rights>1998 INIST-CNRS</rights><rights>Copyright 1998 Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c265t-2664b7c002eb8d8bac82ce7015777e1ca9f95cf505efb7d3559c42901c6a04a23</citedby><cites>FETCH-LOGICAL-c265t-2664b7c002eb8d8bac82ce7015777e1ca9f95cf505efb7d3559c42901c6a04a23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2213978$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9518667$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HATTON, C. J</creatorcontrib><creatorcontrib>PEERS, C</creatorcontrib><title>Arachidonic acid inhibits both K+ and Ca2+ currents in isolated type I cells of the rat carotid body</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>Whole-cell patch-clamp recordings were used to investigate the effects of arachidonic acid (AA) on K+ and Ca2+ channels in isolated rat type I carotid body cells. AA (2-20 microM) produced a concentration-dependent inhibition of both K+ currents and Ca2+ channel currents. The effects of AA on K+ currents were unaffected by indomethacin (5 microM), phenidone (5 microM) or 1-aminobenzotriazole (3 mM), suggesting that AA did not exert its effects via cyclo-oxygenase, lipoxygenase or cytochrome P-450 (cP-450) metabolism. Our results suggest that AA directly and non-selectively inhibits ionic currents in rat type I carotid body cells.</description><subject>Animals</subject><subject>Arachidonic Acid - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Calcium Channel Blockers - pharmacology</subject><subject>Calcium Channels - metabolism</subject><subject>Cardiorespiratory control. Arterial mecano- and chemoreceptor</subject><subject>Carotid Body - drug effects</subject><subject>Carotid Body - enzymology</subject><subject>Carotid Body - metabolism</subject><subject>Cyclooxygenase Inhibitors - pharmacology</subject><subject>Cytochrome P-450 Enzyme Inhibitors</subject><subject>Electrophysiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Lipoxygenase Inhibitors - pharmacology</subject><subject>Patch-Clamp Techniques</subject><subject>Potassium Channel Blockers</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Vertebrates: respiratory system</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kM1LJDEQxcOi6KzunyDkILKL9JqkO19HGfZDVvCweg7VlTQT6emMSeYw_709OsypeLz3qoofIVec_eSMq7v_jDHVGGvb71b_YLwzs_pCFtxo0SjRsROyOEbOyddSXmfZtpadkTMruVFKL4i_z4Cr6NMUkQJGT-O0in2shfaprui_WwqTp0sQtxS3OYdpduJEY0kj1OBp3W0CfaAYxrHQNNC6CjRDpQg51Xldn_zukpwOMJbw7TAvyMvvX8_Lv83j05-H5f1jg0LJ2gilul4jYyL0xpse0AgMmnGptQ4cwQ5W4iCZDEOvfSulxU5YxlEB60C0F-Tmc-8mp7dtKNWtY9l_BlNI2-K01aplRs1B-RnEnErJYXCbHNeQd44zt6frPui6PTpntfug68zcuzoc2Pbr4I-tA87Zvz74UBDGIcOEsRxjQvDWatO-A6hAgTM</recordid><startdate>19980323</startdate><enddate>19980323</enddate><creator>HATTON, C. J</creator><creator>PEERS, C</creator><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19980323</creationdate><title>Arachidonic acid inhibits both K+ and Ca2+ currents in isolated type I cells of the rat carotid body</title><author>HATTON, C. J ; PEERS, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c265t-2664b7c002eb8d8bac82ce7015777e1ca9f95cf505efb7d3559c42901c6a04a23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Arachidonic Acid - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Calcium Channel Blockers - pharmacology</topic><topic>Calcium Channels - metabolism</topic><topic>Cardiorespiratory control. Arterial mecano- and chemoreceptor</topic><topic>Carotid Body - drug effects</topic><topic>Carotid Body - enzymology</topic><topic>Carotid Body - metabolism</topic><topic>Cyclooxygenase Inhibitors - pharmacology</topic><topic>Cytochrome P-450 Enzyme Inhibitors</topic><topic>Electrophysiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Lipoxygenase Inhibitors - pharmacology</topic><topic>Patch-Clamp Techniques</topic><topic>Potassium Channel Blockers</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Vertebrates: respiratory system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HATTON, C. J</creatorcontrib><creatorcontrib>PEERS, C</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HATTON, C. J</au><au>PEERS, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Arachidonic acid inhibits both K+ and Ca2+ currents in isolated type I cells of the rat carotid body</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1998-03-23</date><risdate>1998</risdate><volume>787</volume><issue>2</issue><spage>315</spage><epage>320</epage><pages>315-320</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>Whole-cell patch-clamp recordings were used to investigate the effects of arachidonic acid (AA) on K+ and Ca2+ channels in isolated rat type I carotid body cells. AA (2-20 microM) produced a concentration-dependent inhibition of both K+ currents and Ca2+ channel currents. The effects of AA on K+ currents were unaffected by indomethacin (5 microM), phenidone (5 microM) or 1-aminobenzotriazole (3 mM), suggesting that AA did not exert its effects via cyclo-oxygenase, lipoxygenase or cytochrome P-450 (cP-450) metabolism. Our results suggest that AA directly and non-selectively inhibits ionic currents in rat type I carotid body cells.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier</pub><pmid>9518667</pmid><doi>10.1016/S0006-8993(97)01486-8</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-8993 |
| ispartof | Brain research, 1998-03, Vol.787 (2), p.315-320 |
| issn | 0006-8993 1872-6240 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79763086 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Arachidonic Acid - pharmacology Biological and medical sciences Calcium Channel Blockers - pharmacology Calcium Channels - metabolism Cardiorespiratory control. Arterial mecano- and chemoreceptor Carotid Body - drug effects Carotid Body - enzymology Carotid Body - metabolism Cyclooxygenase Inhibitors - pharmacology Cytochrome P-450 Enzyme Inhibitors Electrophysiology Fundamental and applied biological sciences. Psychology Lipoxygenase Inhibitors - pharmacology Patch-Clamp Techniques Potassium Channel Blockers Rats Rats, Wistar Vertebrates: respiratory system |
| title | Arachidonic acid inhibits both K+ and Ca2+ currents in isolated type I cells of the rat carotid body |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T09%3A04%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Arachidonic%20acid%20inhibits%20both%20K+%20and%20Ca2+%20currents%20in%20isolated%20type%20I%20cells%20of%20the%20rat%20carotid%20body&rft.jtitle=Brain%20research&rft.au=HATTON,%20C.%20J&rft.date=1998-03-23&rft.volume=787&rft.issue=2&rft.spage=315&rft.epage=320&rft.pages=315-320&rft.issn=0006-8993&rft.eissn=1872-6240&rft.coden=BRREAP&rft_id=info:doi/10.1016/S0006-8993(97)01486-8&rft_dat=%3Cproquest_cross%3E79763086%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=79763086&rft_id=info:pmid/9518667&rfr_iscdi=true |