Arachidonic acid inhibits both K+ and Ca2+ currents in isolated type I cells of the rat carotid body

Whole-cell patch-clamp recordings were used to investigate the effects of arachidonic acid (AA) on K+ and Ca2+ channels in isolated rat type I carotid body cells. AA (2-20 microM) produced a concentration-dependent inhibition of both K+ currents and Ca2+ channel currents. The effects of AA on K+ cur...

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Veröffentlicht in:	Brain research 1998-03, Vol.787 (2), p.315-320
Hauptverfasser:	HATTON, C. J, PEERS, C
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Arachidonic Acid - pharmacology Biological and medical sciences Calcium Channel Blockers - pharmacology Calcium Channels - metabolism Cardiorespiratory control. Arterial mecano- and chemoreceptor Carotid Body - drug effects Carotid Body - enzymology Carotid Body - metabolism Cyclooxygenase Inhibitors - pharmacology Cytochrome P-450 Enzyme Inhibitors Electrophysiology Fundamental and applied biological sciences. Psychology Lipoxygenase Inhibitors - pharmacology Patch-Clamp Techniques Potassium Channel Blockers Rats Rats, Wistar Vertebrates: respiratory system
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Zusammenfassung:	Whole-cell patch-clamp recordings were used to investigate the effects of arachidonic acid (AA) on K+ and Ca2+ channels in isolated rat type I carotid body cells. AA (2-20 microM) produced a concentration-dependent inhibition of both K+ currents and Ca2+ channel currents. The effects of AA on K+ currents were unaffected by indomethacin (5 microM), phenidone (5 microM) or 1-aminobenzotriazole (3 mM), suggesting that AA did not exert its effects via cyclo-oxygenase, lipoxygenase or cytochrome P-450 (cP-450) metabolism. Our results suggest that AA directly and non-selectively inhibits ionic currents in rat type I carotid body cells.
ISSN:	0006-8993 1872-6240
DOI:	10.1016/S0006-8993(97)01486-8