Autocrine effect of endothelin on DNA synthesis in human vascular endothelial cells

To elucidate the role of endogenous endothelin on DNA synthesis in endothelial cells, we have investigated the effects of rabbit anti-ET γ globulin on DNA synthesis in cultured human vascular endothelial cells. DNA synthesis was markedly inhibited by anti-ET γ globulin in a concentration dependent m...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biochemical and biophysical research communications 1990-04, Vol.168 (2), p.537-543
Hauptverfasser:	Takagi, Yasuyuki, Fukase, Masaaki, Takata, Shoichiro, Yoshimi, Hiroki, Tokunaga, Osamu, Fujita, Takuo
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Cell physiology Cells, Cultured DNA - biosynthesis Endothelins Endothelium, Vascular - drug effects Endothelium, Vascular - metabolism Fundamental and applied biological sciences. Psychology gamma-Globulins - genetics Growth Substances Humans Ionomycin - metabolism Molecular and cellular biology Peptides - pharmacology Rabbits Radioimmunoassay Responses to growth factors, tumor promotors, other factors Thrombin - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	To elucidate the role of endogenous endothelin on DNA synthesis in endothelial cells, we have investigated the effects of rabbit anti-ET γ globulin on DNA synthesis in cultured human vascular endothelial cells. DNA synthesis was markedly inhibited by anti-ET γ globulin in a concentration dependent manner in the presence of fetal calf serum(FCS) (x5000; 34%, x2500; 54%, x1000; 70%), but not in the absence of FCS. These data suggest that endogenous ET modulates FCS-stimulated DNA synthesis in an autocrine fashion.
ISSN:	0006-291X 1090-2104
DOI:	10.1016/0006-291X(90)92354-3