Sequence location of a putative transglutaminase cross-linking site in human vitronectin

We attempted to locate the glutamine residue in human vitronectin, susceptible to cross-linking by transglutaminases. Vitronectin was incubated with 14C-labelled putrescine and plasma factor XIIIa and, after reduction and alkylation, the vitronectin was digested with trypsin. HPLC of the digest foll...

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Veröffentlicht in:FEBS letters 1990-03, Vol.262 (2), p.269-274
Hauptverfasser: Skorstengaard, Karna, Halkier, Torben, Højrup, Peter, Mosher, Deane
Format: Artikel
Sprache:eng
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Zusammenfassung:We attempted to locate the glutamine residue in human vitronectin, susceptible to cross-linking by transglutaminases. Vitronectin was incubated with 14C-labelled putrescine and plasma factor XIIIa and, after reduction and alkylation, the vitronectin was digested with trypsin. HPLC of the digest followed by scintillation counting revealed one major and two minor radioactivity labelled peaks. Sub-digestion with Staphylococcus aureus protease, sequence analysis and mass-spectrometry of the resulting peptides demonstrated that Gln-93 of vitronectin had incorporated putrescine. Additionally, Gln-73, Gln-84 and Gln-86 were found to be minor sites for incorporation.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(90)80208-Z