Inhibition of alpha-aminoisobutyric acid uptake by mastoparan in Madin-Darby Canine Kidney cells

Mastoparan, a wasp venom, was found to inhibit Na +− dependent net alpha-aminoisobutyric acid (AIB) uptake in Madin Darby Canine Kidney (MDCK) cells. Mastoparan also produced a significant increase in AIB efflux when compared to controls. Pretreatment of MDCK cells with 2 mM neomycin attenuated mast...

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Veröffentlicht in:	Life sciences (1973) 1990, Vol.46 (12), p.865-873
Hauptverfasser:	Eng, Samuel P., Clough, David L., Lo, Chu S.
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha -aminoisobutyric acid Aminoisobutyric Acids - metabolism Animal poisons toxicology. Antivenoms Animals Bee Venoms - pharmacology Biological and medical sciences Biological Transport, Active - drug effects Cell Line Dose-Response Relationship, Drug Kinetics mastoparan Medical sciences Neomycin - pharmacology Ouabain - pharmacology Peptides Sodium - pharmacology Toxicology Wasp Venoms - pharmacology
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Beschreibung
Zusammenfassung:	Mastoparan, a wasp venom, was found to inhibit Na +− dependent net alpha-aminoisobutyric acid (AIB) uptake in Madin Darby Canine Kidney (MDCK) cells. Mastoparan also produced a significant increase in AIB efflux when compared to controls. Pretreatment of MDCK cells with 2 mM neomycin attenuated mastoparan's inhibition of net AIB uptake and completely suppressed mastoparan-mediated increases in AIB efflux when compared to controls. These data suggest that mastoparan's inhibition of net AIB uptake involves more than a single basic mechanism.
ISSN:	0024-3205 1879-0631
DOI:	10.1016/0024-3205(90)90116-9