Collagen and Elastin Metabolism in Hypertensive Pulmonary Arteries of Rats
We evaluated the processes controlling the accumulation of collagen and elastin in main pulmonary arteries of rats during an episode of hypoxic pulmonary hypertension. Explant cultures of main pulmonary arteries were incubated with [H]proline to measure collagen and protein synthesis and percent col...
Gespeichert in:
| Veröffentlicht in: | Circulation research 1990-04, Vol.66 (4), p.968-978 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 978 |
|---|---|
| container_issue | 4 |
| container_start_page | 968 |
| container_title | Circulation research |
| container_volume | 66 |
| creator | Poiani, George J Tozzi, Carol A Yohn, Susan E Pierce, Richard A Belsky, Sandra A Berg, Richard A Yu, Shiu Y Deak, Susan B Riley, David J |
| description | We evaluated the processes controlling the accumulation of collagen and elastin in main pulmonary arteries of rats during an episode of hypoxic pulmonary hypertension. Explant cultures of main pulmonary arteries were incubated with [H]proline to measure collagen and protein synthesis and percent collagen synthesis. Elastin synthesis was measured by [C] valine incorporation into insoluble elastin. Relative collagen synthesis increased twofold (from 1.1±0.2×10 to 2.0±1.0×10 disintegrations per minute [C]hydroxyproline/vessel/hr/mg protein), relative collagen synthesis doubled (from 2% to 4–5% of total protein synthesis), and elastin synthesis increased ninefold (from 0.4±0.2×10 to 3.6±0.6×10 dpm [C]valine/vessel/hr/mg protein) in early hypertension. The level of pro αl(I) collagen RNA paralleled the relative collagen synthetic rate during the study period. Within 7 days of recovery from hypoxia, collagen and elastin contents were normal. We conclude that collagen and elastin in main pulmonary arteries are synthesized rapidly during an episode of hypoxic pulmonary hypertension and that collagen and elastin are rapidly removed from the hypertensive vessel during normoxic recovery. |
| doi_str_mv | 10.1161/01.RES.66.4.968 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79687832</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>79687832</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4439-ee3766e05d233f4c96233a42a24855bf1b2fc622dc9729b2706e66d2e76cc5113</originalsourceid><addsrcrecordid>eNo9kU1v2zAMQIWhQ5Z1O-9UwIdiN7vUh6XoWATp0iJDi3Q7C7JMt95kO5PsBvn3U5CgJ4LkI0E9EfKNQkGppDdAi-3quZCyEIWWiw9kTksmclEqekHmAKBzxTl8Ip9j_ANABWd6RmaMU7XQak4eloP39gX7zPZ1tvI2jm2f_cTRVoNvY5elbH3YYRixj-0bZk-T74behkN2m2qhxZgNTba1Y_xCPjbWR_x6jpfk993q13Kdbx5_3C9vN7kTgusckSspEcqacd4Ip2WKVjDLxKIsq4ZWrHGSsdppxXTFFEiUsmaopHMlpfySfD_t3YXh34RxNF0bHaZn9DhM0ajkQS04S-DNCXRhiDFgY3ah7dLphoI52jNATbJnpDTCpKk0cXVePVUd1u_8WVfqX5_7Njrrm2B718Z3rATBBDteKE7YfvDJUfzrpz0G84rWj68mfQpwoCynWgOIlOXHkub_ASbhhds</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>79687832</pqid></control><display><type>article</type><title>Collagen and Elastin Metabolism in Hypertensive Pulmonary Arteries of Rats</title><source>MEDLINE</source><source>American Heart Association Journals</source><source>Journals@Ovid Ovid Autoload</source><source>EZB Electronic Journals Library</source><creator>Poiani, George J ; Tozzi, Carol A ; Yohn, Susan E ; Pierce, Richard A ; Belsky, Sandra A ; Berg, Richard A ; Yu, Shiu Y ; Deak, Susan B ; Riley, David J</creator><creatorcontrib>Poiani, George J ; Tozzi, Carol A ; Yohn, Susan E ; Pierce, Richard A ; Belsky, Sandra A ; Berg, Richard A ; Yu, Shiu Y ; Deak, Susan B ; Riley, David J</creatorcontrib><description>We evaluated the processes controlling the accumulation of collagen and elastin in main pulmonary arteries of rats during an episode of hypoxic pulmonary hypertension. Explant cultures of main pulmonary arteries were incubated with [H]proline to measure collagen and protein synthesis and percent collagen synthesis. Elastin synthesis was measured by [C] valine incorporation into insoluble elastin. Relative collagen synthesis increased twofold (from 1.1±0.2×10 to 2.0±1.0×10 disintegrations per minute [C]hydroxyproline/vessel/hr/mg protein), relative collagen synthesis doubled (from 2% to 4–5% of total protein synthesis), and elastin synthesis increased ninefold (from 0.4±0.2×10 to 3.6±0.6×10 dpm [C]valine/vessel/hr/mg protein) in early hypertension. The level of pro αl(I) collagen RNA paralleled the relative collagen synthetic rate during the study period. Within 7 days of recovery from hypoxia, collagen and elastin contents were normal. We conclude that collagen and elastin in main pulmonary arteries are synthesized rapidly during an episode of hypoxic pulmonary hypertension and that collagen and elastin are rapidly removed from the hypertensive vessel during normoxic recovery.</description><identifier>ISSN: 0009-7330</identifier><identifier>EISSN: 1524-4571</identifier><identifier>DOI: 10.1161/01.RES.66.4.968</identifier><identifier>PMID: 2317897</identifier><identifier>CODEN: CIRUAL</identifier><language>eng</language><publisher>Hagerstown, MD: American Heart Association, Inc</publisher><subject>Actins - genetics ; Animals ; Biological and medical sciences ; Cardiology. Vascular system ; Chronic cor pulmonale ; Collagen - metabolism ; Elastin - metabolism ; Heart ; Hypertension - metabolism ; Hypertension - physiopathology ; Medical sciences ; Pneumology ; Procollagen - genetics ; Proteins - metabolism ; Pulmonary Artery - metabolism ; Pulmonary hypertension. Acute cor pulmonale. Pulmonary embolism. Pulmonary vascular diseases ; Rats ; Rats, Inbred Strains ; RNA, Messenger - metabolism</subject><ispartof>Circulation research, 1990-04, Vol.66 (4), p.968-978</ispartof><rights>1990 American Heart Association, Inc.</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4439-ee3766e05d233f4c96233a42a24855bf1b2fc622dc9729b2706e66d2e76cc5113</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,3688,27928,27929</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5042421$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2317897$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Poiani, George J</creatorcontrib><creatorcontrib>Tozzi, Carol A</creatorcontrib><creatorcontrib>Yohn, Susan E</creatorcontrib><creatorcontrib>Pierce, Richard A</creatorcontrib><creatorcontrib>Belsky, Sandra A</creatorcontrib><creatorcontrib>Berg, Richard A</creatorcontrib><creatorcontrib>Yu, Shiu Y</creatorcontrib><creatorcontrib>Deak, Susan B</creatorcontrib><creatorcontrib>Riley, David J</creatorcontrib><title>Collagen and Elastin Metabolism in Hypertensive Pulmonary Arteries of Rats</title><title>Circulation research</title><addtitle>Circ Res</addtitle><description>We evaluated the processes controlling the accumulation of collagen and elastin in main pulmonary arteries of rats during an episode of hypoxic pulmonary hypertension. Explant cultures of main pulmonary arteries were incubated with [H]proline to measure collagen and protein synthesis and percent collagen synthesis. Elastin synthesis was measured by [C] valine incorporation into insoluble elastin. Relative collagen synthesis increased twofold (from 1.1±0.2×10 to 2.0±1.0×10 disintegrations per minute [C]hydroxyproline/vessel/hr/mg protein), relative collagen synthesis doubled (from 2% to 4–5% of total protein synthesis), and elastin synthesis increased ninefold (from 0.4±0.2×10 to 3.6±0.6×10 dpm [C]valine/vessel/hr/mg protein) in early hypertension. The level of pro αl(I) collagen RNA paralleled the relative collagen synthetic rate during the study period. Within 7 days of recovery from hypoxia, collagen and elastin contents were normal. We conclude that collagen and elastin in main pulmonary arteries are synthesized rapidly during an episode of hypoxic pulmonary hypertension and that collagen and elastin are rapidly removed from the hypertensive vessel during normoxic recovery.</description><subject>Actins - genetics</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cardiology. Vascular system</subject><subject>Chronic cor pulmonale</subject><subject>Collagen - metabolism</subject><subject>Elastin - metabolism</subject><subject>Heart</subject><subject>Hypertension - metabolism</subject><subject>Hypertension - physiopathology</subject><subject>Medical sciences</subject><subject>Pneumology</subject><subject>Procollagen - genetics</subject><subject>Proteins - metabolism</subject><subject>Pulmonary Artery - metabolism</subject><subject>Pulmonary hypertension. Acute cor pulmonale. Pulmonary embolism. Pulmonary vascular diseases</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>RNA, Messenger - metabolism</subject><issn>0009-7330</issn><issn>1524-4571</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kU1v2zAMQIWhQ5Z1O-9UwIdiN7vUh6XoWATp0iJDi3Q7C7JMt95kO5PsBvn3U5CgJ4LkI0E9EfKNQkGppDdAi-3quZCyEIWWiw9kTksmclEqekHmAKBzxTl8Ip9j_ANABWd6RmaMU7XQak4eloP39gX7zPZ1tvI2jm2f_cTRVoNvY5elbH3YYRixj-0bZk-T74behkN2m2qhxZgNTba1Y_xCPjbWR_x6jpfk993q13Kdbx5_3C9vN7kTgusckSspEcqacd4Ip2WKVjDLxKIsq4ZWrHGSsdppxXTFFEiUsmaopHMlpfySfD_t3YXh34RxNF0bHaZn9DhM0ajkQS04S-DNCXRhiDFgY3ah7dLphoI52jNATbJnpDTCpKk0cXVePVUd1u_8WVfqX5_7Njrrm2B718Z3rATBBDteKE7YfvDJUfzrpz0G84rWj68mfQpwoCynWgOIlOXHkub_ASbhhds</recordid><startdate>199004</startdate><enddate>199004</enddate><creator>Poiani, George J</creator><creator>Tozzi, Carol A</creator><creator>Yohn, Susan E</creator><creator>Pierce, Richard A</creator><creator>Belsky, Sandra A</creator><creator>Berg, Richard A</creator><creator>Yu, Shiu Y</creator><creator>Deak, Susan B</creator><creator>Riley, David J</creator><general>American Heart Association, Inc</general><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199004</creationdate><title>Collagen and Elastin Metabolism in Hypertensive Pulmonary Arteries of Rats</title><author>Poiani, George J ; Tozzi, Carol A ; Yohn, Susan E ; Pierce, Richard A ; Belsky, Sandra A ; Berg, Richard A ; Yu, Shiu Y ; Deak, Susan B ; Riley, David J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4439-ee3766e05d233f4c96233a42a24855bf1b2fc622dc9729b2706e66d2e76cc5113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Actins - genetics</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cardiology. Vascular system</topic><topic>Chronic cor pulmonale</topic><topic>Collagen - metabolism</topic><topic>Elastin - metabolism</topic><topic>Heart</topic><topic>Hypertension - metabolism</topic><topic>Hypertension - physiopathology</topic><topic>Medical sciences</topic><topic>Pneumology</topic><topic>Procollagen - genetics</topic><topic>Proteins - metabolism</topic><topic>Pulmonary Artery - metabolism</topic><topic>Pulmonary hypertension. Acute cor pulmonale. Pulmonary embolism. Pulmonary vascular diseases</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Poiani, George J</creatorcontrib><creatorcontrib>Tozzi, Carol A</creatorcontrib><creatorcontrib>Yohn, Susan E</creatorcontrib><creatorcontrib>Pierce, Richard A</creatorcontrib><creatorcontrib>Belsky, Sandra A</creatorcontrib><creatorcontrib>Berg, Richard A</creatorcontrib><creatorcontrib>Yu, Shiu Y</creatorcontrib><creatorcontrib>Deak, Susan B</creatorcontrib><creatorcontrib>Riley, David J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Circulation research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Poiani, George J</au><au>Tozzi, Carol A</au><au>Yohn, Susan E</au><au>Pierce, Richard A</au><au>Belsky, Sandra A</au><au>Berg, Richard A</au><au>Yu, Shiu Y</au><au>Deak, Susan B</au><au>Riley, David J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Collagen and Elastin Metabolism in Hypertensive Pulmonary Arteries of Rats</atitle><jtitle>Circulation research</jtitle><addtitle>Circ Res</addtitle><date>1990-04</date><risdate>1990</risdate><volume>66</volume><issue>4</issue><spage>968</spage><epage>978</epage><pages>968-978</pages><issn>0009-7330</issn><eissn>1524-4571</eissn><coden>CIRUAL</coden><abstract>We evaluated the processes controlling the accumulation of collagen and elastin in main pulmonary arteries of rats during an episode of hypoxic pulmonary hypertension. Explant cultures of main pulmonary arteries were incubated with [H]proline to measure collagen and protein synthesis and percent collagen synthesis. Elastin synthesis was measured by [C] valine incorporation into insoluble elastin. Relative collagen synthesis increased twofold (from 1.1±0.2×10 to 2.0±1.0×10 disintegrations per minute [C]hydroxyproline/vessel/hr/mg protein), relative collagen synthesis doubled (from 2% to 4–5% of total protein synthesis), and elastin synthesis increased ninefold (from 0.4±0.2×10 to 3.6±0.6×10 dpm [C]valine/vessel/hr/mg protein) in early hypertension. The level of pro αl(I) collagen RNA paralleled the relative collagen synthetic rate during the study period. Within 7 days of recovery from hypoxia, collagen and elastin contents were normal. We conclude that collagen and elastin in main pulmonary arteries are synthesized rapidly during an episode of hypoxic pulmonary hypertension and that collagen and elastin are rapidly removed from the hypertensive vessel during normoxic recovery.</abstract><cop>Hagerstown, MD</cop><pub>American Heart Association, Inc</pub><pmid>2317897</pmid><doi>10.1161/01.RES.66.4.968</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0009-7330 |
| ispartof | Circulation research, 1990-04, Vol.66 (4), p.968-978 |
| issn | 0009-7330 1524-4571 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79687832 |
| source | MEDLINE; American Heart Association Journals; Journals@Ovid Ovid Autoload; EZB Electronic Journals Library |
| subjects | Actins - genetics Animals Biological and medical sciences Cardiology. Vascular system Chronic cor pulmonale Collagen - metabolism Elastin - metabolism Heart Hypertension - metabolism Hypertension - physiopathology Medical sciences Pneumology Procollagen - genetics Proteins - metabolism Pulmonary Artery - metabolism Pulmonary hypertension. Acute cor pulmonale. Pulmonary embolism. Pulmonary vascular diseases Rats Rats, Inbred Strains RNA, Messenger - metabolism |
| title | Collagen and Elastin Metabolism in Hypertensive Pulmonary Arteries of Rats |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-16T21%3A23%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Collagen%20and%20Elastin%20Metabolism%20in%20Hypertensive%20Pulmonary%20Arteries%20of%20Rats&rft.jtitle=Circulation%20research&rft.au=Poiani,%20George%20J&rft.date=1990-04&rft.volume=66&rft.issue=4&rft.spage=968&rft.epage=978&rft.pages=968-978&rft.issn=0009-7330&rft.eissn=1524-4571&rft.coden=CIRUAL&rft_id=info:doi/10.1161/01.RES.66.4.968&rft_dat=%3Cproquest_cross%3E79687832%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=79687832&rft_id=info:pmid/2317897&rfr_iscdi=true |