Collagen and Elastin Metabolism in Hypertensive Pulmonary Arteries of Rats

We evaluated the processes controlling the accumulation of collagen and elastin in main pulmonary arteries of rats during an episode of hypoxic pulmonary hypertension. Explant cultures of main pulmonary arteries were incubated with [H]proline to measure collagen and protein synthesis and percent col...

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Veröffentlicht in:Circulation research 1990-04, Vol.66 (4), p.968-978
Hauptverfasser: Poiani, George J, Tozzi, Carol A, Yohn, Susan E, Pierce, Richard A, Belsky, Sandra A, Berg, Richard A, Yu, Shiu Y, Deak, Susan B, Riley, David J
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Sprache:eng
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Zusammenfassung:We evaluated the processes controlling the accumulation of collagen and elastin in main pulmonary arteries of rats during an episode of hypoxic pulmonary hypertension. Explant cultures of main pulmonary arteries were incubated with [H]proline to measure collagen and protein synthesis and percent collagen synthesis. Elastin synthesis was measured by [C] valine incorporation into insoluble elastin. Relative collagen synthesis increased twofold (from 1.1±0.2×10 to 2.0±1.0×10 disintegrations per minute [C]hydroxyproline/vessel/hr/mg protein), relative collagen synthesis doubled (from 2% to 4–5% of total protein synthesis), and elastin synthesis increased ninefold (from 0.4±0.2×10 to 3.6±0.6×10 dpm [C]valine/vessel/hr/mg protein) in early hypertension. The level of pro αl(I) collagen RNA paralleled the relative collagen synthetic rate during the study period. Within 7 days of recovery from hypoxia, collagen and elastin contents were normal. We conclude that collagen and elastin in main pulmonary arteries are synthesized rapidly during an episode of hypoxic pulmonary hypertension and that collagen and elastin are rapidly removed from the hypertensive vessel during normoxic recovery.
ISSN:0009-7330
1524-4571
DOI:10.1161/01.RES.66.4.968