The sarcolemmal Ca2+-ATPase of the ischemic-reperfused myocardium: protective effect of hypocalcemia on calmodulin-stimulated activity
This study was designed to examine the role of calcium in the ischemia-induced changes of calmodulin-stimulated Ca2+-ATPase activity of heart sarcolemma of dogs subjected to coronary artery ligation (90 min) and reperfusion (30 min). This was attained by the application of systemic hemodialysis with...
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| Veröffentlicht in: | Life sciences (1973) 1998, Vol.62 (1), p.29-36 |
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| creator | Samouilidou, E C Karli, J N Levis, G M Darsinos, J T |
| description | This study was designed to examine the role of calcium in the ischemia-induced changes of calmodulin-stimulated Ca2+-ATPase activity of heart sarcolemma of dogs subjected to coronary artery ligation (90 min) and reperfusion (30 min). This was attained by the application of systemic hemodialysis with low Ca2+ dialysate in six dogs (group A) and the comparison of the results with those obtained from animals subjected to normal Ca2+ hemodialysis (control group B, n=7). A very significant (p |
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This was attained by the application of systemic hemodialysis with low Ca2+ dialysate in six dogs (group A) and the comparison of the results with those obtained from animals subjected to normal Ca2+ hemodialysis (control group B, n=7). A very significant (p<0.001) decrease was found in the calmodulin-stimulated Ca2+-ATPase activity measured in the ischemic and non-ischemic parts of group B. This was associated with a decrease in the maximal velocity (v(max)) of the reaction of stimulation and an increase in the apparent Km for calmodulin. The kinetics of the calmodulin-stimulated Ca2+-ATPase also assessed in the presence of trifluoroperazine, a specific inhibitor for calmodulin binding, showed that the affinity for calmodulin was higher in the ischemic part of group A than of B, while v(max) was not substantially different. The above data may suggest that the inhibition of the calmodulin-stimulated Ca2+-ATPase produced by ischemia-reperfusion and its preservation under low Ca2+, are exerted at the calmodulin-binding site of the enzyme.</description><identifier>ISSN: 0024-3205</identifier><identifier>PMID: 9444965</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Animals ; Calcium-Transporting ATPases - drug effects ; Calcium-Transporting ATPases - metabolism ; Calmodulin - metabolism ; Calmodulin - pharmacology ; Dogs ; Female ; Heart - drug effects ; Hypocalcemia - physiopathology ; Male ; Myocardial Ischemia - metabolism ; Myocardial Reperfusion ; Myocardium - enzymology ; Sarcolemma - drug effects ; Sarcolemma - enzymology</subject><ispartof>Life sciences (1973), 1998, Vol.62 (1), p.29-36</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4009</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9444965$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Samouilidou, E C</creatorcontrib><creatorcontrib>Karli, J N</creatorcontrib><creatorcontrib>Levis, G M</creatorcontrib><creatorcontrib>Darsinos, J T</creatorcontrib><title>The sarcolemmal Ca2+-ATPase of the ischemic-reperfused myocardium: protective effect of hypocalcemia on calmodulin-stimulated activity</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>This study was designed to examine the role of calcium in the ischemia-induced changes of calmodulin-stimulated Ca2+-ATPase activity of heart sarcolemma of dogs subjected to coronary artery ligation (90 min) and reperfusion (30 min). This was attained by the application of systemic hemodialysis with low Ca2+ dialysate in six dogs (group A) and the comparison of the results with those obtained from animals subjected to normal Ca2+ hemodialysis (control group B, n=7). A very significant (p<0.001) decrease was found in the calmodulin-stimulated Ca2+-ATPase activity measured in the ischemic and non-ischemic parts of group B. This was associated with a decrease in the maximal velocity (v(max)) of the reaction of stimulation and an increase in the apparent Km for calmodulin. The kinetics of the calmodulin-stimulated Ca2+-ATPase also assessed in the presence of trifluoroperazine, a specific inhibitor for calmodulin binding, showed that the affinity for calmodulin was higher in the ischemic part of group A than of B, while v(max) was not substantially different. The above data may suggest that the inhibition of the calmodulin-stimulated Ca2+-ATPase produced by ischemia-reperfusion and its preservation under low Ca2+, are exerted at the calmodulin-binding site of the enzyme.</description><subject>Animals</subject><subject>Calcium-Transporting ATPases - drug effects</subject><subject>Calcium-Transporting ATPases - metabolism</subject><subject>Calmodulin - metabolism</subject><subject>Calmodulin - pharmacology</subject><subject>Dogs</subject><subject>Female</subject><subject>Heart - drug effects</subject><subject>Hypocalcemia - physiopathology</subject><subject>Male</subject><subject>Myocardial Ischemia - metabolism</subject><subject>Myocardial Reperfusion</subject><subject>Myocardium - enzymology</subject><subject>Sarcolemma - drug effects</subject><subject>Sarcolemma - enzymology</subject><issn>0024-3205</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNotkEFLxDAQhXtQ1nX1Jwg5eZFCnDSt9bYs6goLeui9zCYTNpJsapMK_QP-brO4p3kw33vMm4tiyTlUpQAur4rrGL8451I2YlEs2qqq2loui9_uQCziqIIj79GxDcJDue4-MRILhqW8tlEdyFtVjjTQaKZImvk5KBy1nfwzG8aQSCX7Q4yMyepkPMxDJpzKRmThyLL2QU_OHsuYrJ8cphyDJ5tN801xadBFuj3PVdG9vnSbbbn7eHvfrHflIIUs0TxyMKgrAKOkEW3dyIbzvZJ7VGSAqlbV3GjKNZ8aaACgEZqwhkwrAWJV3P_H5pO_J4qp97kcOYdHClPsm7auJQDP4N0ZnPaedD-M1uM49-e_iT-deGtg</recordid><startdate>1998</startdate><enddate>1998</enddate><creator>Samouilidou, E C</creator><creator>Karli, J N</creator><creator>Levis, G M</creator><creator>Darsinos, J T</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>1998</creationdate><title>The sarcolemmal Ca2+-ATPase of the ischemic-reperfused myocardium: protective effect of hypocalcemia on calmodulin-stimulated activity</title><author>Samouilidou, E C ; Karli, J N ; Levis, G M ; Darsinos, J T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p535-af102fad422fc5f39675700bc5bacef2e49c60fde055872722273dea62c5fc323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Calcium-Transporting ATPases - drug effects</topic><topic>Calcium-Transporting ATPases - metabolism</topic><topic>Calmodulin - metabolism</topic><topic>Calmodulin - pharmacology</topic><topic>Dogs</topic><topic>Female</topic><topic>Heart - drug effects</topic><topic>Hypocalcemia - physiopathology</topic><topic>Male</topic><topic>Myocardial Ischemia - metabolism</topic><topic>Myocardial Reperfusion</topic><topic>Myocardium - enzymology</topic><topic>Sarcolemma - drug effects</topic><topic>Sarcolemma - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Samouilidou, E C</creatorcontrib><creatorcontrib>Karli, J N</creatorcontrib><creatorcontrib>Levis, G M</creatorcontrib><creatorcontrib>Darsinos, J T</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Samouilidou, E C</au><au>Karli, J N</au><au>Levis, G M</au><au>Darsinos, J T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The sarcolemmal Ca2+-ATPase of the ischemic-reperfused myocardium: protective effect of hypocalcemia on calmodulin-stimulated activity</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>1998</date><risdate>1998</risdate><volume>62</volume><issue>1</issue><spage>29</spage><epage>36</epage><pages>29-36</pages><issn>0024-3205</issn><abstract>This study was designed to examine the role of calcium in the ischemia-induced changes of calmodulin-stimulated Ca2+-ATPase activity of heart sarcolemma of dogs subjected to coronary artery ligation (90 min) and reperfusion (30 min). This was attained by the application of systemic hemodialysis with low Ca2+ dialysate in six dogs (group A) and the comparison of the results with those obtained from animals subjected to normal Ca2+ hemodialysis (control group B, n=7). A very significant (p<0.001) decrease was found in the calmodulin-stimulated Ca2+-ATPase activity measured in the ischemic and non-ischemic parts of group B. This was associated with a decrease in the maximal velocity (v(max)) of the reaction of stimulation and an increase in the apparent Km for calmodulin. The kinetics of the calmodulin-stimulated Ca2+-ATPase also assessed in the presence of trifluoroperazine, a specific inhibitor for calmodulin binding, showed that the affinity for calmodulin was higher in the ischemic part of group A than of B, while v(max) was not substantially different. The above data may suggest that the inhibition of the calmodulin-stimulated Ca2+-ATPase produced by ischemia-reperfusion and its preservation under low Ca2+, are exerted at the calmodulin-binding site of the enzyme.</abstract><cop>Netherlands</cop><pmid>9444965</pmid><tpages>8</tpages></addata></record> |
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| ispartof | Life sciences (1973), 1998, Vol.62 (1), p.29-36 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Calcium-Transporting ATPases - drug effects Calcium-Transporting ATPases - metabolism Calmodulin - metabolism Calmodulin - pharmacology Dogs Female Heart - drug effects Hypocalcemia - physiopathology Male Myocardial Ischemia - metabolism Myocardial Reperfusion Myocardium - enzymology Sarcolemma - drug effects Sarcolemma - enzymology |
| title | The sarcolemmal Ca2+-ATPase of the ischemic-reperfused myocardium: protective effect of hypocalcemia on calmodulin-stimulated activity |
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