The hepatic biosynthesis of rat thyroxine binding globulin (TBG): Demonstration, ontogenesis, and UP-regulation in experimental hypothyroidism

The hepatic biosynthesis of rat thyroxine binding globulin (TBG): Demonstration, ontogenesis, and UP-regulation in experimental hypothyroidism

Using a human thyroxine binding globulin (TBG) cDNA probe, we demonstrate that rat liver contains two TBG mRNA species of different length, consisting of about 1.8 Kb and 2.4 Kb respectively. Slot blot analysis of the hepatic mRNAs from rats of different age reveals a fair correlation between the de...

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Veröffentlicht in:Biochemical and biophysical research communications 1990-02, Vol.167 (1), p.317-322
Hauptverfasser: Vranckx, Roger, Rouaze, Marielle, Savu, Lia, Nunez, Emmanuel A., Beaumont, Carole, Flink, Irwin L.
Format: Artikel
Sprache:eng
Schlagworte:
Analytical, structural and metabolic biochemistry
Animals
Binding and carrier proteins
Biological and medical sciences
Blotting, Northern
DNA - genetics
DNA Probes
Fundamental and applied biological sciences. Psychology
Humans
Hypothyroidism - genetics
Hypothyroidism - metabolism
Liver - metabolism
Nucleic Acid Hybridization
Proteins
Rats
Rats, Inbred Strains
RNA, Messenger - analysis
Thyroid Gland - physiology
Thyroidectomy
Thyroxine-Binding Proteins - biosynthesis
Thyroxine-Binding Proteins - genetics
Thyroxine-Binding Proteins - metabolism
Up-Regulation
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Zusammenfassung:Using a human thyroxine binding globulin (TBG) cDNA probe, we demonstrate that rat liver contains two TBG mRNA species of different length, consisting of about 1.8 Kb and 2.4 Kb respectively. Slot blot analysis of the hepatic mRNAs from rats of different age reveals a fair correlation between the developmental trend of the messengers and that of the TBG circulating levels. Finally Northern blot and slot blot studies demonstrate that the increase of serum TBG induced in adults by thyroidectomy actually reflects an enhanced hepatic biosynthesis of the protein.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(90)91767-M