Further characterization, isolation and identification of the epithelial cell‐surface antigen defined by monoclonal antibody aua1

The human epithelial antigen recognized by monoclonal antibody (MAb) AUAl has been characterized as a cell‐surface glycoprotein. It has been isolated from human colonic mucosa by AUAl affinity separation. N‐terminal peptide sequence of this purified material has revealed a 17 amino acid sequence which identifies it with one of a group of similar epithelial/tumour‐associated glycoproteins defined by a variety of MAbs. Using the polymerase chain reaction to map the gene encoding this antigen, our previous AUAl antigen assignment to chromosome 2 has been confirmed.