Primary structure and functional expression from complementary DNA of a human interleukin-1 receptor antagonist

Human monocytes induced with adherent IgG secrete an interleukin-1 receptor antagonist which could be important for the in vivo regulation of IL-1 activity. A complementary DNA for this molecule has been isolated from a human monocyte library. Analysis of monocyte RNA indicates that the gene is tran...

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Veröffentlicht in:	Nature (London) 1990-01, Vol.343 (6256), p.341-346
Hauptverfasser:	Eisenberg, Stephen P., Evans, Ron J., Arend, William P., Verderber, Evie, Brewer, Michael T, Hannum, Charles H., Thompson, Robert C.
Format:	Artikel
Sprache:	eng
Schlagworte:	3' Untranslated regions Amino Acid Sequence Amino acids Arthritis Base Sequence Biochemistry Chemical compounds Cloning Complementary DNA Cytokines Deoxyribonucleic acid Dinoprostone - biosynthesis Disease DNA DNA - genetics DNA - isolation & purification E coli Escherichia coli Escherichia coli - genetics Fibroblasts - metabolism Gene Expression Genetics Glycosylation Homology Humanities and Social Sciences Humans Immunity (Disease) Inflammatory diseases Interleukin 1 Interleukin 1 receptor antagonist Interleukin 1 Receptor Antagonist Protein Interleukin-1 - genetics Interleukin-1 - pharmacology Medical research Methionine Molecular Sequence Data Monocytes - metabolism mRNA multidisciplinary Nucleic Acid Hybridization Peptides Pharmacology Proteins Proteins - genetics Proteins - pharmacology Receptors, Immunologic - antagonists & inhibitors Receptors, Interleukin-1 Recombinant Proteins - genetics Recombinant Proteins - pharmacology Rheumatoid arthritis RNA, Messenger - analysis RNA, Messenger - biosynthesis Science Science (multidisciplinary) Sequence Homology, Nucleic Acid Sialoglycoproteins
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Beschreibung
Zusammenfassung:	Human monocytes induced with adherent IgG secrete an interleukin-1 receptor antagonist which could be important for the in vivo regulation of IL-1 activity. A complementary DNA for this molecule has been isolated from a human monocyte library. Analysis of monocyte RNA indicates that the gene is transcriptionally regulated. The sequence of the receptor antagonist indicates that it is structurally similar to IL-lp. Expression of the cDNA in Escherichia coli yields IL-1 receptor antagonist activity.
ISSN:	0028-0836 1476-4687
DOI:	10.1038/343341a0