Philanthotoxins. A mass spectrometric investigation

A method employing liquid secondary‐ion mass spectrometry (SIMS) in conjunction with metastable‐ion measurements (linked scanning at constant B/E) to obtain sequence‐specific information for three synthetic polyamine isomers was developed. The normal liquid SIMS spectra gave molecular weight information, but important sequence ions were of low intensity or obscured by the background. The metastable‐ion spectra contained important fragment ions in particular due to cleavage along the polyamine chain. One of the three synthetic isomers was identical with a toxin present in the venom of the digger wasp. In conjunction with nuclear magnetic resonance spectroscopic studies, this should be a powerful method for the structural characterization of other closely related toxins present in the venom of this wasp.