Latex immunoassay for rapid detection of Newcastle disease virus

A rapid test has been developed based on the technique of latex immunoassay for the detection of Newcastle disease virus from suspected tissue suspensions. The latex particles were sensitised with globulins and were used for antigen detection. Of the 258 samples tested, 165 samples were positive by...

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Veröffentlicht in:	Tropical animal health and production 1997-11, Vol.29 (4), p.227-230
Hauptverfasser:	Thirumurugan, G, Jayakumar, R, Kumanan, K, Venugopalan, A.T, Nachimuthu, K
Format:	Artikel
Sprache:	eng
Schlagworte:	accuracy Animals Brain - virology Chickens Disease Outbreaks - veterinary Gastrointestinal Contents - microbiology hemagglutination inhibition test Hemagglutination Tests Ileocecal Valve - virology Immunoassay - methods latex agglutination test Latex Fixation Tests - veterinary Newcastle Disease - diagnosis Newcastle Disease - epidemiology Newcastle disease virus Newcastle disease virus - isolation & purification rapid methods Spleen - virology tissues Trachea - virology
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Beschreibung
Zusammenfassung:	A rapid test has been developed based on the technique of latex immunoassay for the detection of Newcastle disease virus from suspected tissue suspensions. The latex particles were sensitised with globulins and were used for antigen detection. Of the 258 samples tested, 165 samples were positive by this kit which was compared for its efficacy with the standard OIE approved haemagglutination (HA) and haemagglutination inhibition (HI) tests. No significant difference (P > 0.05) was observed between the tests. The sensitivity and specificity of the developed test was 94.19% and 87.63% respectively.
ISSN:	0049-4747 1573-7438
DOI:	10.1007/BF02632309