Plus/minus screening of rabbit corneal endothelial cDNA library

Plus/minus screening of the rabbit corneal cDNA library was performed using corneal and iris RNA as probes. Thirteen clones were isolated: three ferritin H-chains, a NADH-ubiquinone oxidoreductase B22 subunit, an alpha 1 type VIII collagen, a 25 KDa FKBP-506 binding protein (FKBP25), a thrombospondi...

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Veröffentlicht in:	Japanese journal of ophthalmology 1997-11, Vol.41 (6), p.370-375
Hauptverfasser:	Hotta, Yoshihiro, Kitagawa, Hitoshi, Fujiki, Keiko, Fujimaki, Takuro, Ohnuki, Hisashi, Sakuma, Hitoshi, Iwata, Fumino, Watanabe, Michifumi, Nakayasu, Kiyoo, Kanai, Atsushi
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Base Sequence Biological and medical sciences Carrier Proteins - genetics Cattle cDNA Cell Adhesion Molecules - genetics Clone Cells Collagen - genetics cornea DNA, Complementary - analysis Electrophoresis, Agar Gel Endothelium, Corneal Eye and associated structures. Visual pathways and centers. Vision Eye Proteins - genetics ferritin Ferritins - genetics Fundamental and applied biological sciences. Psychology Gene Library Humans Molecular Sequence Data NAD(P)H Dehydrogenase (Quinone) - genetics NADH-ubiquinone oxidoreductase Rabbits Sequence Analysis, DNA Tacrolimus Binding Proteins thrombospondin Thrombospondins - genetics Vertebrates: nervous system and sense organs
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Zusammenfassung:	Plus/minus screening of the rabbit corneal cDNA library was performed using corneal and iris RNA as probes. Thirteen clones were isolated: three ferritin H-chains, a NADH-ubiquinone oxidoreductase B22 subunit, an alpha 1 type VIII collagen, a 25 KDa FKBP-506 binding protein (FKBP25), a thrombospondin 2, and six unknown clones. Although proteins translocated from these isolated mRNA are not corneal specific, they play an important role in the cornea. None of the isolated known mRNAs maps to chromosome 1, 16, or 20. These clones, thrombospondin excepted, were not observed in the high frequency clones in the profile of the aortic endothelial cDNA library.
ISSN:	0021-5155 1613-2246
DOI:	10.1016/S0021-5155(97)00086-5