Screening for selected trace elements in single cell populations by energy dispersive X-ray fluorescence spectrometry

Screening for selected trace elements in single cell populations by energy dispersive X-ray fluorescence spectrometry

An adaptation of the thin film approach to energy dispersive X-ray fluorescence spectrometry (EDXRF) is described, which provides for a screening analysis for Fe, Ni, Cu, Zn, Se, and Cd in K-562 leukemic cells incubated in various metal-enhanced cultures. Valuable quantitative and semiquantitative d...

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Veröffentlicht in:Biological trace element research 1989-12, Vol.23 (1), p.65-75
Hauptverfasser: Bray, J T, Maier, R H, Pories, W J
Format: Artikel
Sprache:eng
Schlagworte:
Cells, Cultured
Membranes, Artificial
Reference Standards
Spectrometry, X-Ray Emission
Trace Elements - analysis
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Zusammenfassung:An adaptation of the thin film approach to energy dispersive X-ray fluorescence spectrometry (EDXRF) is described, which provides for a screening analysis for Fe, Ni, Cu, Zn, Se, and Cd in K-562 leukemic cells incubated in various metal-enhanced cultures. Valuable quantitative and semiquantitative data can be obtained on as few as 400,000 cells.
ISSN:0163-4984
1559-0720
DOI:10.1007/BF02917178