Metallothionein induction in fetal rat brain and neonatal primary astrocyte cultures by in utero exposure to elemental mercury vapor (Hg 0)

Brain metallothionein (MT) protein and mRNA levels were determined in the fetal rat following in utero (gestational days 7–21) exposure to elemental mercury vapor (Hg 0; 300 μg Hg/m 3; 4 h/day). Total RNA was probed on northern blots with [ α- 32P]dCTP-labeled synthetic cDNA probes specific for rat...

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Veröffentlicht in:	Brain research 1997-12, Vol.778 (1), p.222-232
Hauptverfasser:	Aschner, M, Lorscheider, F.L, Cowan, K.S, Conklin, D.R, Vimy, M.J, Lash, L.H
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Animals, Newborn Astrocyte Astrocytes - drug effects Astrocytes - metabolism Biomarker Blotting, Northern Blotting, Western Brain Brain - drug effects Brain - embryology Brain - metabolism Cells, Cultured Embryonic and Fetal Development - drug effects Female Gestational Age Glutathione - metabolism In utero Mercury - pharmacology Mercury vapor Hg 0 Metallothionein Metallothionein - biosynthesis Nerve Tissue Proteins - biosynthesis Pregnancy Prenatal Exposure Delayed Effects Rat Rats Rats, Sprague-Dawley
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Zusammenfassung:	Brain metallothionein (MT) protein and mRNA levels were determined in the fetal rat following in utero (gestational days 7–21) exposure to elemental mercury vapor (Hg 0; 300 μg Hg/m 3; 4 h/day). Total RNA was probed on northern blots with [ α- 32P]dCTP-labeled synthetic cDNA probes specific for rat MT isoform mRNAs. The probes for MT-I and MT-II mRNA hybridized to a single band of approximately 550 and 450 nucleotides, respectively. Expression of whole brain MT-I mRNA in full-term fetal rats (day 21) was significantly increased ( P
ISSN:	0006-8993 1872-6240
DOI:	10.1016/S0006-8993(97)01095-0