Establishment of a new human pancreatic adenocarcinoma cell line, MDAPanc-3

A new cell line was established from a liver metastasis of a human pancreatic adenocarcinoma. The cell line, MDAPanc-3, which arose from a moderately differentiated adenocarcinoma, produces carbonic anhydrase II mRNA, but no detectable levels of insulin or alpha amylase mRNA. The stem line chromosom...

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Veröffentlicht in:	Pancreas 1990, Vol.5 (1), p.8-16
Hauptverfasser:	FRAZIER, M. L, PATHAK, S, ZU-WEI WANG, CLEARY, K, SINGLETARY, S. E, OLIVE, M, MACKAY, B, STECK, P. A, LEVIN, B
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenocarcinoma - pathology alpha-Amylases - genetics Biological and medical sciences Blotting, Northern Cell Division - drug effects Gastroenterology. Liver. Pancreas. Abdomen Humans Insulin - genetics Karyotyping Keratins - genetics Liver. Biliary tract. Portal circulation. Exocrine pancreas Male Medical sciences Middle Aged Molecular Weight Pancreatic Neoplasms - pathology Protein Biosynthesis RNA, Neoplasm - genetics Sincalide - analogs & derivatives Sincalide - pharmacology Tumor Cells, Cultured Tumors
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Zusammenfassung:	A new cell line was established from a liver metastasis of a human pancreatic adenocarcinoma. The cell line, MDAPanc-3, which arose from a moderately differentiated adenocarcinoma, produces carbonic anhydrase II mRNA, but no detectable levels of insulin or alpha amylase mRNA. The stem line chromosome number was determined to be 43, with six marker chromosomes. Growth of MDAPanc-3 is stimulated by cholecystokinin (CCK) fragment 26-33. The cell line will be useful in further studies on the mechanism(s) by which CCK stimulates growth of certain human pancreatic adenocarcinomas and normal human pancreatic exocrine tissue.
ISSN:	0885-3177 1536-4828
DOI:	10.1097/00006676-199001000-00002