Intra-amygdala infusion of 2-chloroadenosine suppresses amygdala-kindled seizures
The seizure-modulating effects of 2-chloroadenosine (2-CLA) infused directly into the amygdala were investigated. Different groups of amygdala-kindled rats were infused (1 μl) with 2-CLA (0.25, 1, 10 and 25 nM), caffeine (200 μM and 2 mM), a combination of the two or artificial cerebrospinal fluid (...
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| Veröffentlicht in: | Brain research 1997-11, Vol.775 (1-2), p.37-42 |
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| creator | Pourgholami, M.H Rostampour, M Mirnajafi-Zadeh, J Palizvan, M.R |
| description | The seizure-modulating effects of 2-chloroadenosine (2-CLA) infused directly into the amygdala were investigated. Different groups of amygdala-kindled rats were infused (1 μl) with 2-CLA (0.25, 1, 10 and 25 nM), caffeine (200 μM and 2 mM), a combination of the two or artificial cerebrospinal fluid (ACSF) applied directly through a cannula located in the amygdala. Infusion of 2-CLA dramatically suppressed seizure stage (SS), after discharge duration (ADD) and stage 5 seizure duration (S5D), while the latency to bilateral forelimb clonus (S4L) was significantly prolonged. These anticonvulsant effects were evident after 5 min, reached a maximum at the 60 min time point and were still detectable 360 min post-2-CLA infusion. Pretreatment with caffeine blocked the anticonvulsant effects of 2-CLA dose-dependently. These results may suggest that in amygdaloid-kindled rats, adenosine receptors located in the amygdala play a major role in the expression of the anticonvulsant activity of 2-CLA. |
| doi_str_mv | 10.1016/S0006-8993(97)00769-5 |
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Different groups of amygdala-kindled rats were infused (1 μl) with 2-CLA (0.25, 1, 10 and 25 nM), caffeine (200 μM and 2 mM), a combination of the two or artificial cerebrospinal fluid (ACSF) applied directly through a cannula located in the amygdala. Infusion of 2-CLA dramatically suppressed seizure stage (SS), after discharge duration (ADD) and stage 5 seizure duration (S5D), while the latency to bilateral forelimb clonus (S4L) was significantly prolonged. These anticonvulsant effects were evident after 5 min, reached a maximum at the 60 min time point and were still detectable 360 min post-2-CLA infusion. Pretreatment with caffeine blocked the anticonvulsant effects of 2-CLA dose-dependently. 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Different groups of amygdala-kindled rats were infused (1 μl) with 2-CLA (0.25, 1, 10 and 25 nM), caffeine (200 μM and 2 mM), a combination of the two or artificial cerebrospinal fluid (ACSF) applied directly through a cannula located in the amygdala. Infusion of 2-CLA dramatically suppressed seizure stage (SS), after discharge duration (ADD) and stage 5 seizure duration (S5D), while the latency to bilateral forelimb clonus (S4L) was significantly prolonged. These anticonvulsant effects were evident after 5 min, reached a maximum at the 60 min time point and were still detectable 360 min post-2-CLA infusion. Pretreatment with caffeine blocked the anticonvulsant effects of 2-CLA dose-dependently. These results may suggest that in amygdaloid-kindled rats, adenosine receptors located in the amygdala play a major role in the expression of the anticonvulsant activity of 2-CLA.</description><subject>2-Chloroadenosine</subject><subject>2-Chloroadenosine - administration & dosage</subject><subject>2-Chloroadenosine - pharmacology</subject><subject>Adenosine A1 receptor</subject><subject>Amygdala</subject><subject>Amygdala - physiology</subject><subject>Animals</subject><subject>Anticonvulsants - pharmacology</subject><subject>Caffeine</subject><subject>Caffeine - pharmacology</subject><subject>Central Nervous System Stimulants - pharmacology</subject><subject>Injections</subject><subject>Kindling</subject><subject>Kindling, Neurologic - drug effects</subject><subject>Male</subject><subject>Purinergic P1 Receptor Antagonists</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Seizure</subject><subject>Seizures - prevention & control</subject><subject>Time Factors</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LxDAQhoMo67r6E4SeRA_RfLVJTiLiFwgi6jlkk6lG22ZNWkF_vXV39eppGN7nnYEHoX1Kjimh1ckDIaTCSmt-qOURIbLSuNxAU6okwxUTZBNN_5BttJPz67hyrskETbTgWrFqiu5vuj5ZbNvPZ28bW4SuHnKIXRHrgmH30sQUrYcu5tBBkYfFIkHOkIvfBn4LnW_AFxnC1zCGu2irtk2GvfWcoafLi8fza3x7d3VzfnaLHde0x-AkI0J7SbiTSikPvoaqdExYqAUwYoUreem8F0RTReeCOkuFdbV1pSOSz9DB6u4ixfcBcm_akB00je0gDtlIXTItlP4XpBUnTGk2guUKdCnmnKA2ixRamz4NJebHuVk6Nz9CjZZm6dyUY29__WCYt-D_WmvJY366ymHU8REgmewCdA58SOB642P458M3RBSShg</recordid><startdate>19971114</startdate><enddate>19971114</enddate><creator>Pourgholami, M.H</creator><creator>Rostampour, M</creator><creator>Mirnajafi-Zadeh, J</creator><creator>Palizvan, M.R</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19971114</creationdate><title>Intra-amygdala infusion of 2-chloroadenosine suppresses amygdala-kindled seizures</title><author>Pourgholami, M.H ; Rostampour, M ; Mirnajafi-Zadeh, J ; Palizvan, M.R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-ec72049d703c7888dedfe65c24aef4e20a4c535cdd409181b41ca14acfac5c073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>2-Chloroadenosine</topic><topic>2-Chloroadenosine - administration & dosage</topic><topic>2-Chloroadenosine - pharmacology</topic><topic>Adenosine A1 receptor</topic><topic>Amygdala</topic><topic>Amygdala - physiology</topic><topic>Animals</topic><topic>Anticonvulsants - pharmacology</topic><topic>Caffeine</topic><topic>Caffeine - pharmacology</topic><topic>Central Nervous System Stimulants - pharmacology</topic><topic>Injections</topic><topic>Kindling</topic><topic>Kindling, Neurologic - drug effects</topic><topic>Male</topic><topic>Purinergic P1 Receptor Antagonists</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Seizure</topic><topic>Seizures - prevention & control</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pourgholami, M.H</creatorcontrib><creatorcontrib>Rostampour, M</creatorcontrib><creatorcontrib>Mirnajafi-Zadeh, J</creatorcontrib><creatorcontrib>Palizvan, M.R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pourgholami, M.H</au><au>Rostampour, M</au><au>Mirnajafi-Zadeh, J</au><au>Palizvan, M.R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intra-amygdala infusion of 2-chloroadenosine suppresses amygdala-kindled seizures</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1997-11-14</date><risdate>1997</risdate><volume>775</volume><issue>1-2</issue><spage>37</spage><epage>42</epage><pages>37-42</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><abstract>The seizure-modulating effects of 2-chloroadenosine (2-CLA) infused directly into the amygdala were investigated. Different groups of amygdala-kindled rats were infused (1 μl) with 2-CLA (0.25, 1, 10 and 25 nM), caffeine (200 μM and 2 mM), a combination of the two or artificial cerebrospinal fluid (ACSF) applied directly through a cannula located in the amygdala. Infusion of 2-CLA dramatically suppressed seizure stage (SS), after discharge duration (ADD) and stage 5 seizure duration (S5D), while the latency to bilateral forelimb clonus (S4L) was significantly prolonged. These anticonvulsant effects were evident after 5 min, reached a maximum at the 60 min time point and were still detectable 360 min post-2-CLA infusion. Pretreatment with caffeine blocked the anticonvulsant effects of 2-CLA dose-dependently. These results may suggest that in amygdaloid-kindled rats, adenosine receptors located in the amygdala play a major role in the expression of the anticonvulsant activity of 2-CLA.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>9439826</pmid><doi>10.1016/S0006-8993(97)00769-5</doi><tpages>6</tpages></addata></record> |
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| subjects | 2-Chloroadenosine 2-Chloroadenosine - administration & dosage 2-Chloroadenosine - pharmacology Adenosine A1 receptor Amygdala Amygdala - physiology Animals Anticonvulsants - pharmacology Caffeine Caffeine - pharmacology Central Nervous System Stimulants - pharmacology Injections Kindling Kindling, Neurologic - drug effects Male Purinergic P1 Receptor Antagonists Rats Rats, Sprague-Dawley Seizure Seizures - prevention & control Time Factors |
| title | Intra-amygdala infusion of 2-chloroadenosine suppresses amygdala-kindled seizures |
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