Expression cloning and characterization of a renal organic anion transporter from winter flounder

The cDNA coding for a renal p-aminohippurate (PAH) transporter from winter flounder ( Pseudopleuronectes americanus), designated fROAT, was cloned by functional expression in Xenopus laevis oocytes. fROAT is approximately 2.8 kbp in length and encodes a protein of 562 amino acids, related to the rat...

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Veröffentlicht in:	FEBS letters 1997-11, Vol.417 (3), p.287-291
Hauptverfasser:	Wolff, N.A, Werner, A, Burkhardt, S, Burckhardt, G
Format:	Artikel
Sprache:	eng
Schlagworte:	4-acetamido-4′-isothiocyanatostilbene-2,2′-disulfonate Amino Acid Sequence Animals Anion Transport Proteins Base Sequence Carrier Proteins - biosynthesis Carrier Proteins - chemistry Carrier Proteins - pharmacology casein kinase II CK-2 Cloning, Molecular Expression cloning Female Flounder Gene Library glutarate Kidney Kidney - metabolism Kinetics Molecular Sequence Data Oocytes - physiology Organic anion transport p-Aminohippurate p-Aminohippuric Acid - pharmacokinetics PAH PKA PKC protein kinase A protein kinase C Pseudopleuronectes americanus Rats RNA, Messenger - isolation & purification RNA, Messenger - metabolism Sequence Alignment Sequence Homology, Amino Acid SITS TEA tetraethylammonium Xenopus laevis
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Beschreibung
Zusammenfassung:	The cDNA coding for a renal p-aminohippurate (PAH) transporter from winter flounder ( Pseudopleuronectes americanus), designated fROAT, was cloned by functional expression in Xenopus laevis oocytes. fROAT is approximately 2.8 kbp in length and encodes a protein of 562 amino acids, related to the rat renal organic anion transporter ROAT1/OAT1 and the organic cation transporters OCT1 and OCT2. In oocytes, fROAT mediated probenecid-sensitive PAH uptake, with a K m for PAH of about 20 μM, and inhibited by external glutarate (GA) (1 mM). The functional characteristics suggest that fROAT is the basolateral PAH/dicarboxylate exchanger of the flounder kidney.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/S0014-5793(97)01304-5