Construction and characterization of versatile kanamycin-resistance cassettes derived from the Tn5 transposon

Construction and characterization of versatile kanamycin-resistance cassettes derived from the Tn5 transposon

We have developed plasmids with the Tn5 kanamycin-resistance gene (kan) flanked either symmetrically or asymmetrically by several restriction sites. These can be used to provide a selectable genetic marker or to mobilize restriction sites and sense or nonsense codons into genes. The 1.3-kb kan casse...

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Veröffentlicht in:Gene 1989-12, Vol.85 (1), p.205-207
Hauptverfasser: MUSSO, R. E, HODAM, T
Format: Artikel
Sprache:eng
Schlagworte:
Base Sequence
Biological and medical sciences
Biotechnology
Codon
DNA Transposable Elements
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Kanamycin Resistance - genetics
Methods. Procedures. Technologies
Molecular Sequence Data
Oligonucleotide Probes
Restriction Mapping
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Zusammenfassung:We have developed plasmids with the Tn5 kanamycin-resistance gene (kan) flanked either symmetrically or asymmetrically by several restriction sites. These can be used to provide a selectable genetic marker or to mobilize restriction sites and sense or nonsense codons into genes. The 1.3-kb kan cassette exhibits polarity effects in both directions.
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(89)90482-4