Cloning and sequence analysis of the fermentative alcohol-dehydrogenase-encoding gene of Escherichia coli
A 6-kb fragment of DNA, which complemented defects in the alcohol dehydrogenase (ADH)-encoding gene ( adhE) of Escherichia coli, was cloned into a multicopy vector. Both ADH and coenzyme-A-linked acetaldehyde dehydrogenase (ACDH) activities were encoded by the plasmid, pHIL8. The adhE gene was ident...
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| Veröffentlicht in: | Gene 1989-12, Vol.85 (1), p.209-214 |
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| container_title | Gene |
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| creator | Goodlove, Paige E. Cunningham, Philip R. Parker, Jack Clark, David P. |
| description | A 6-kb fragment of DNA, which complemented defects in the alcohol dehydrogenase (ADH)-encoding gene (
adhE) of
Escherichia coli, was cloned into a multicopy vector. Both ADH and coenzyme-A-linked acetaldehyde dehydrogenase (ACDH) activities were encoded by the plasmid, pHIL8. The
adhE gene was identified as an open reading frame of 891 codons encoding an
M
r 96 008 protein (minus the initiating methionine). Codon usage analysis indicates that
adhE should be highly expressed. This gene shows no significant homology to any previously sequenced ADH-encoding gene. |
| doi_str_mv | 10.1016/0378-1119(89)90483-6 |
| format | Article |
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adhE) of
Escherichia coli, was cloned into a multicopy vector. Both ADH and coenzyme-A-linked acetaldehyde dehydrogenase (ACDH) activities were encoded by the plasmid, pHIL8. The
adhE gene was identified as an open reading frame of 891 codons encoding an
M
r 96 008 protein (minus the initiating methionine). Codon usage analysis indicates that
adhE should be highly expressed. This gene shows no significant homology to any previously sequenced ADH-encoding gene.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(89)90483-6</identifier><identifier>PMID: 2695398</identifier><identifier>CODEN: GENED6</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Acetaldehyde ; Alcohol Dehydrogenase - genetics ; Amino Acid Sequence ; anaerobic growth ; Base Sequence ; Biological and medical sciences ; Cloning, Molecular ; Codon - genetics ; codon usage ; coenzyme A ; Cosmids ; DNA, Bacterial - genetics ; Escherichia coli - enzymology ; Escherichia coli - genetics ; Fermentation ; Fundamental and applied biological sciences. Psychology ; Gene Library ; Genes, Bacterial ; Genes. Genome ; Genetic Vectors ; Genotype ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Molecular Weight ; recombinant DNA ; Restriction Mapping ; Sequence Homology, Nucleic Acid</subject><ispartof>Gene, 1989-12, Vol.85 (1), p.209-214</ispartof><rights>1989</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-4ebe45d047ed13321b8e6c1750ab7bd5ff8b4259849b3375602e134e3dba9373</citedby><cites>FETCH-LOGICAL-c452t-4ebe45d047ed13321b8e6c1750ab7bd5ff8b4259849b3375602e134e3dba9373</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(89)90483-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6723042$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2695398$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Goodlove, Paige E.</creatorcontrib><creatorcontrib>Cunningham, Philip R.</creatorcontrib><creatorcontrib>Parker, Jack</creatorcontrib><creatorcontrib>Clark, David P.</creatorcontrib><title>Cloning and sequence analysis of the fermentative alcohol-dehydrogenase-encoding gene of Escherichia coli</title><title>Gene</title><addtitle>Gene</addtitle><description>A 6-kb fragment of DNA, which complemented defects in the alcohol dehydrogenase (ADH)-encoding gene (
adhE) of
Escherichia coli, was cloned into a multicopy vector. Both ADH and coenzyme-A-linked acetaldehyde dehydrogenase (ACDH) activities were encoded by the plasmid, pHIL8. The
adhE gene was identified as an open reading frame of 891 codons encoding an
M
r 96 008 protein (minus the initiating methionine). Codon usage analysis indicates that
adhE should be highly expressed. This gene shows no significant homology to any previously sequenced ADH-encoding gene.</description><subject>Acetaldehyde</subject><subject>Alcohol Dehydrogenase - genetics</subject><subject>Amino Acid Sequence</subject><subject>anaerobic growth</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>Codon - genetics</subject><subject>codon usage</subject><subject>coenzyme A</subject><subject>Cosmids</subject><subject>DNA, Bacterial - genetics</subject><subject>Escherichia coli - enzymology</subject><subject>Escherichia coli - genetics</subject><subject>Fermentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Library</subject><subject>Genes, Bacterial</subject><subject>Genes. Genome</subject><subject>Genetic Vectors</subject><subject>Genotype</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Molecular Weight</subject><subject>recombinant DNA</subject><subject>Restriction Mapping</subject><subject>Sequence Homology, Nucleic Acid</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9LHDEYh0Np0a32GyjMQUp7GM3fSXIRymKrIHjxHjLJO04kO9FkVthv30x32WNzCS-_5_eSPAhdEHxNMOluMJOqJYToH0r_1Jgr1naf0IooqVuMmfqMVkfkFH0t5RXXIwQ9QSe004JptUJhHdMUppfGTr4p8L6FyUEdbNyVUJo0NPMIzQB5A9Ns5_BRw-jSmGLrYdz5nF5gsgXa2kt-WVRnWHp3xY2QgxuDbVyK4Rx9GWws8O1wn6Hn33fP6_v28enPw_rXY-u4oHPLoQcuPOYSPGGMkl5B54gU2Pay92IYVM-p0IrrnjEpOkyBMA7M91Yzyc7Q9_3at5zqb8psNqE4iNFOkLbFSM0lo4JXkO9Bl1MpGQbzlsPG5p0h2CyCzWLPLPaM0uafYNPV2uVh_7bfgD-WDkZrfnXIbXE2DtlOLpQj1knKMKcVu91jUFV8BMimuLC49yGDm41P4f_v-AsEyJfk</recordid><startdate>19891221</startdate><enddate>19891221</enddate><creator>Goodlove, Paige E.</creator><creator>Cunningham, Philip R.</creator><creator>Parker, Jack</creator><creator>Clark, David P.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19891221</creationdate><title>Cloning and sequence analysis of the fermentative alcohol-dehydrogenase-encoding gene of Escherichia coli</title><author>Goodlove, Paige E. ; Cunningham, Philip R. ; Parker, Jack ; Clark, David P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-4ebe45d047ed13321b8e6c1750ab7bd5ff8b4259849b3375602e134e3dba9373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Acetaldehyde</topic><topic>Alcohol Dehydrogenase - genetics</topic><topic>Amino Acid Sequence</topic><topic>anaerobic growth</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>Codon - genetics</topic><topic>codon usage</topic><topic>coenzyme A</topic><topic>Cosmids</topic><topic>DNA, Bacterial - genetics</topic><topic>Escherichia coli - enzymology</topic><topic>Escherichia coli - genetics</topic><topic>Fermentation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Library</topic><topic>Genes, Bacterial</topic><topic>Genes. Genome</topic><topic>Genetic Vectors</topic><topic>Genotype</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Molecular Weight</topic><topic>recombinant DNA</topic><topic>Restriction Mapping</topic><topic>Sequence Homology, Nucleic Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Goodlove, Paige E.</creatorcontrib><creatorcontrib>Cunningham, Philip R.</creatorcontrib><creatorcontrib>Parker, Jack</creatorcontrib><creatorcontrib>Clark, David P.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Goodlove, Paige E.</au><au>Cunningham, Philip R.</au><au>Parker, Jack</au><au>Clark, David P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and sequence analysis of the fermentative alcohol-dehydrogenase-encoding gene of Escherichia coli</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1989-12-21</date><risdate>1989</risdate><volume>85</volume><issue>1</issue><spage>209</spage><epage>214</epage><pages>209-214</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><coden>GENED6</coden><abstract>A 6-kb fragment of DNA, which complemented defects in the alcohol dehydrogenase (ADH)-encoding gene (
adhE) of
Escherichia coli, was cloned into a multicopy vector. Both ADH and coenzyme-A-linked acetaldehyde dehydrogenase (ACDH) activities were encoded by the plasmid, pHIL8. The
adhE gene was identified as an open reading frame of 891 codons encoding an
M
r 96 008 protein (minus the initiating methionine). Codon usage analysis indicates that
adhE should be highly expressed. This gene shows no significant homology to any previously sequenced ADH-encoding gene.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>2695398</pmid><doi>10.1016/0378-1119(89)90483-6</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Gene, 1989-12, Vol.85 (1), p.209-214 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79473254 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Acetaldehyde Alcohol Dehydrogenase - genetics Amino Acid Sequence anaerobic growth Base Sequence Biological and medical sciences Cloning, Molecular Codon - genetics codon usage coenzyme A Cosmids DNA, Bacterial - genetics Escherichia coli - enzymology Escherichia coli - genetics Fermentation Fundamental and applied biological sciences. Psychology Gene Library Genes, Bacterial Genes. Genome Genetic Vectors Genotype Molecular and cellular biology Molecular genetics Molecular Sequence Data Molecular Weight recombinant DNA Restriction Mapping Sequence Homology, Nucleic Acid |
| title | Cloning and sequence analysis of the fermentative alcohol-dehydrogenase-encoding gene of Escherichia coli |
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