Cloning and sequence analysis of the fermentative alcohol-dehydrogenase-encoding gene of Escherichia coli

A 6-kb fragment of DNA, which complemented defects in the alcohol dehydrogenase (ADH)-encoding gene ( adhE) of Escherichia coli, was cloned into a multicopy vector. Both ADH and coenzyme-A-linked acetaldehyde dehydrogenase (ACDH) activities were encoded by the plasmid, pHIL8. The adhE gene was ident...

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Veröffentlicht in:	Gene 1989-12, Vol.85 (1), p.209-214
Hauptverfasser:	Goodlove, Paige E., Cunningham, Philip R., Parker, Jack, Clark, David P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetaldehyde Alcohol Dehydrogenase - genetics Amino Acid Sequence anaerobic growth Base Sequence Biological and medical sciences Cloning, Molecular Codon - genetics codon usage coenzyme A Cosmids DNA, Bacterial - genetics Escherichia coli - enzymology Escherichia coli - genetics Fermentation Fundamental and applied biological sciences. Psychology Gene Library Genes, Bacterial Genes. Genome Genetic Vectors Genotype Molecular and cellular biology Molecular genetics Molecular Sequence Data Molecular Weight recombinant DNA Restriction Mapping Sequence Homology, Nucleic Acid
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Beschreibung
Zusammenfassung:	A 6-kb fragment of DNA, which complemented defects in the alcohol dehydrogenase (ADH)-encoding gene ( adhE) of Escherichia coli, was cloned into a multicopy vector. Both ADH and coenzyme-A-linked acetaldehyde dehydrogenase (ACDH) activities were encoded by the plasmid, pHIL8. The adhE gene was identified as an open reading frame of 891 codons encoding an M r 96 008 protein (minus the initiating methionine). Codon usage analysis indicates that adhE should be highly expressed. This gene shows no significant homology to any previously sequenced ADH-encoding gene.
ISSN:	0378-1119 1879-0038
DOI:	10.1016/0378-1119(89)90483-6