Radioimmunoassay to determine postprandial changes in plasma Neuropeptide Y levels in awake dogs

Radioimmunoassay to determine postprandial changes in plasma Neuropeptide Y levels in awake dogs

A specific, precise and sensitive double-antibody radioimmunoassay for Neuropeptide Y (NPY) has been developed. There was no appreciable cross-reactivity with the structurally related peptides, peptide YY (PYY) and pancreatic polypeptide (PP). The minimum detectable plasma NPY level was 3nM. Applica...

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Veröffentlicht in:Neuropeptides (Edinburgh) 1989-10, Vol.14 (3), p.209-212
Hauptverfasser: Balasubramaniam, A., McFadden, D.W., Rudnicki, M., Nussbaum, M.S., Dayal, R., Srivastava, L.S., Fischer, J.E.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cross Reactions
Dogs
Eating
Neuropeptide Y - blood
Neuropeptide Y - metabolism
Neuropeptide Y - standards
Radioimmunoassay - methods
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Zusammenfassung:A specific, precise and sensitive double-antibody radioimmunoassay for Neuropeptide Y (NPY) has been developed. There was no appreciable cross-reactivity with the structurally related peptides, peptide YY (PYY) and pancreatic polypeptide (PP). The minimum detectable plasma NPY level was 3nM. Application of radioimmunoassay to canine models revealed that portal and systemic NPY levels increased significantly following a standard meal.
ISSN:0143-4179
1532-2785
DOI:10.1016/0143-4179(89)90046-2