Propagation and titration of murine cytomegalovirus in a continuous bone marrow-derived stromal cell line (M2-10B4)

Murine cytomegalovirus (MCMV) can only be propagated effectively in mouse embryo fibroblast (MEF) cells. We demonstrate that MCMV replicates significantly better in M2-10B4 cells, a continuous line of murine bone marrow stromal cells. M2-10B4 cells were also comparable to MEF cells for detection of...

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Veröffentlicht in:	Journal of virological methods 1997-11, Vol.68 (2), p.193-198
Hauptverfasser:	Lutarewych, Michael A., Quirk, Mary R., Kringstad, Barbara A., Li, Wuyi, Verfaillie, Catherine M., Jordan, M. Colin
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Bone Marrow Cells - virology Cell Line Cytopathogenic Effect, Viral Female Fundamental and applied biological sciences. Psychology Herpesviridae Infections - virology M2-10B4 cells Mice Mice, Inbred BALB C Mice, Inbred C3H Mice, Inbred C57BL Microbiology Murine cytomegalovirus Muromegalovirus - growth & development Muromegalovirus - isolation & purification Muromegalovirus - physiology Organ Culture Techniques Propagation Spleen - virology Stromal Cells - virology Techniques used in virology Viral Plaque Assay Virology Virus Latency
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Zusammenfassung:	Murine cytomegalovirus (MCMV) can only be propagated effectively in mouse embryo fibroblast (MEF) cells. We demonstrate that MCMV replicates significantly better in M2-10B4 cells, a continuous line of murine bone marrow stromal cells. M2-10B4 cells were also comparable to MEF cells for detection of small amounts of MCMV reactivating from latently infected spleen explants. M2-10B4 cells will be very useful for studies of MCMV pathogenesis.
ISSN:	0166-0934 1879-0984
DOI:	10.1016/S0166-0934(97)00126-2