Effects of recombinant interleukin-1 beta on phospholipase A2 activity, phospholipase A2 mRNA levels, and eicosanoid formation in rabbit chondrocytes

We have investigated the effects of recombinant interleukin-1 beta (rIL-1 beta) on phospholipase A2 activity (PLA2), PLA2 messenger RNA levels, and eicosanoid production in rabbit chondrocytes. Phospholipase A2 activity increased 5 fold with exposure to 1.6 x 10(-11) M rIL-1 beta for 20 hours. An mR...

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Veröffentlicht in:	Biochemical and biophysical research communications 1989-12, Vol.165 (3), p.1079-1084
Hauptverfasser:	KERR, J. S, STEVENS, T. M, DAVIS, G. L, MCLAUGHLIN, J. A, HARRIS, R. R
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Arachidonic Acids - metabolism Biological and medical sciences Cartilage, Articular - enzymology Cell physiology Dinoprost - biosynthesis Dinoprostone - biosynthesis DNA Probes Eicosanoids - biosynthesis Fundamental and applied biological sciences. Psychology Hydroxyeicosatetraenoic Acids - biosynthesis Interleukin-1 - pharmacology Kinetics Leukotriene B4 - biosynthesis Male Molecular and cellular biology Nucleic Acid Hybridization Phospholipases - metabolism Phospholipases A - genetics Phospholipases A - metabolism Phospholipases A2 Prostaglandin D2 - biosynthesis Rabbits Recombinant Proteins Responses to growth factors, tumor promotors, other factors RNA, Messenger - analysis
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Zusammenfassung:	We have investigated the effects of recombinant interleukin-1 beta (rIL-1 beta) on phospholipase A2 activity (PLA2), PLA2 messenger RNA levels, and eicosanoid production in rabbit chondrocytes. Phospholipase A2 activity increased 5 fold with exposure to 1.6 x 10(-11) M rIL-1 beta for 20 hours. An mRNA specific for an extracellular PLA2 was detected by RNA blot hybridization after treatment of the cells with rIL-1 beta. Hydroxylated derivatives of arachidonic acid, including prostaglandins, leukotriene B4, 5-hydroxyeicosa 6E, 8Z, 11Z, 14Z-tetraenoic acid, 12-hydroxyeicosa 5Z, 8Z, 10E, 14Z-tetraenoic acid and 15-hydroxyeicosa 5Z, 8Z, 11Z, 13E-tetraenoic acid increased in cells after rIL-1 beta treatment.
ISSN:	0006-291X 1090-2104
DOI:	10.1016/0006-291X(89)92712-5