DNA-probes for the differentiation of Capnocytophaga species

We designed oligonucleotides to differentiate between the seven currently known Capnocytophaga species. The oligonucleotides were labelled non-radioactively at the 3' end with digoxigenin. The specificity could be demonstrated in a dot-blot hybridization assay by using the type strains, referen...

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Veröffentlicht in:	Molecular and cellular probes 1997-10, Vol.11 (5), p.323-328
Hauptverfasser:	Conrads, G, Mutters, R, Seyfarth, I, Pelz, K
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Typing Techniques Capnocytophaga - classification Capnocytophaga - genetics Digoxigenin DNA Probes DNA, Bacterial - genetics DNA, Ribosomal - genetics Nucleic Acid Hybridization Oligodeoxyribonucleotides - chemistry RNA, Ribosomal, 16S - genetics Sensitivity and Specificity Species Specificity
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Beschreibung
Zusammenfassung:	We designed oligonucleotides to differentiate between the seven currently known Capnocytophaga species. The oligonucleotides were labelled non-radioactively at the 3' end with digoxigenin. The specificity could be demonstrated in a dot-blot hybridization assay by using the type strains, reference strains, and 37 clinical Capnocytophaga isolates as well as 11 representative strains of other taxa as a template. The sensitivity of the assay was calculated with 10(3) bacteria per dot.
ISSN:	0890-8508
DOI:	10.1006/mcpr.1997.0124