Inhibition of invasion of epithelial cells by Tiam1-Rac signaling

Tiam1 encodes an exchange factor for the Rho-like guanosine triphosphatase Rac. Both Tiam1 and activated RacV12 promote invasiveness of T lymphoma cells. In epithelial Madin-Darby canine kidney (MDCK) cells, Tiam1 localized to adherens junctions. Ectopic expression of Tiam1 or RacV12 inhibited hepat...

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Veröffentlicht in:Science (American Association for the Advancement of Science) 1997-11, Vol.278 (5342), p.1464-1466
Hauptverfasser: HORDIJK, P. L, TEN KLOOSTER, J. P, VAN DER KAMMEN, R. A, MICHIELS, F, OOMEN, L. C. J. M, COLLARD, J. G
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Sprache:eng
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Zusammenfassung:Tiam1 encodes an exchange factor for the Rho-like guanosine triphosphatase Rac. Both Tiam1 and activated RacV12 promote invasiveness of T lymphoma cells. In epithelial Madin-Darby canine kidney (MDCK) cells, Tiam1 localized to adherens junctions. Ectopic expression of Tiam1 or RacV12 inhibited hepatocyte growth factor-induced scattering by increasing E-cadherin-mediated cell-cell adhesion accompanied by actin polymerization at cell-cell contacts. In Ras-transformed MDCK cells, expression of Tiam1 or RacV12 restored E-cadherin-mediated adhesion, resulting in phenotypic reversion and loss of invasiveness. These data suggest an invasion-suppressor role for Tiam1 and Rac in epithelial cells.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.278.5342.1464