Effect of PHA-T cell conditioned medium (PHA-TCM) on leukemic cells

Effect of PHA-T cell conditioned medium (PHA-TCM) on leukemic cells

Cord blood T cells, purified by nylon wool column, were incubated with PHA for 18 hours, and the supernatant were harvested as PHA-TCM. The results demonstrated that PHA-TCM could significantly inhibit the growth of U937 cells and reduce their 3H-TdR incorporation and spontaneous colony formation. A...

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Veröffentlicht in:Sheng li hsüeh pao 1989-10, Vol.41 (5), p.476-481
Hauptverfasser: Feng, Y Q, Tang, P H
Format: Artikel
Sprache:chi
Schlagworte:
Cell Differentiation
Culture Media
Humans
Leukemia, Promyelocytic, Acute - pathology
Lymphoma, Large B-Cell, Diffuse - pathology
Phytohemagglutinins - pharmacology
T-Lymphocytes
Tumor Cells, Cultured
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Zusammenfassung:Cord blood T cells, purified by nylon wool column, were incubated with PHA for 18 hours, and the supernatant were harvested as PHA-TCM. The results demonstrated that PHA-TCM could significantly inhibit the growth of U937 cells and reduce their 3H-TdR incorporation and spontaneous colony formation. After 3-6 days of treatment with PHA-TCM in culture, part of U937 cells became macrophage-like, latex-phagocytic, and capable of reducing NBT dye. On the contrary, HL-60 cells did not respond to PHA-TCM inhibition, indicating the selective effect of PHA-TCM.
ISSN:0371-0874