p66 an in vivo target for poly(ADP-ribosyl)ation co-purifies with poly(ADP-ribose) polymerase

A direct immunoassay has been applied for the quantitation of poly(ADP-ribose) polymerase and its automodification in BALB/3T3 (A31) cells. As the cell population reached a high density, a 66 kDa protein (designated p66) which co-purified with the enzyme became highly poly(ADP-ribosyl)ated. Pulse-ch...

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Veröffentlicht in:	Biochemical and biophysical research communications 1989-12, Vol.165 (2), p.631-636
Hauptverfasser:	Zhang, Jingyuan, Ebisuzaki, Kaney
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Diphosphate Ribose - metabolism Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Blotting, Western Cells, Cultured Fundamental and applied biological sciences. Psychology Mice Mice, Inbred BALB C Miscellaneous Molecular Weight Poly(ADP-ribose) Polymerases - isolation & purification Poly(ADP-ribose) Polymerases - metabolism Proteins Proteins - isolation & purification Proteins - metabolism
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Beschreibung
Zusammenfassung:	A direct immunoassay has been applied for the quantitation of poly(ADP-ribose) polymerase and its automodification in BALB/3T3 (A31) cells. As the cell population reached a high density, a 66 kDa protein (designated p66) which co-purified with the enzyme became highly poly(ADP-ribosyl)ated. Pulse-chase experiments as well as a Western blot analysis indicated that the p66 was not a degradation product of poly(ADP-ribose) polymerase.
ISSN:	0006-291X 1090-2104
DOI:	10.1016/S0006-291X(89)80012-9