Cloning of human gene encoding prostaglandin endoperoxide synthase and primary structure of the enzyme

The complete amino acid sequence of human prostaglandin endoperoxide synthase (EC 1.14.99.1, cyclooxygenase) was deduced by cloning and sequence analysis of human genomic DNA coding for the enzyme. The isolated clones covered approximately 40 kilobase pairs of human gene and the protein coding regio...

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Veröffentlicht in:	Biochemical and biophysical research communications 1989-12, Vol.165 (2), p.888-894
Hauptverfasser:	Yokoyama, Chieko, Tanabe, Tadashi
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Base Sequence Biological and medical sciences Cloning, Molecular DNA - genetics Exons Fundamental and applied biological sciences. Psychology Genes Genes. Genome Humans Molecular and cellular biology Molecular genetics Molecular Sequence Data Prostaglandin-Endoperoxide Synthases - genetics Restriction Mapping
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Beschreibung
Zusammenfassung:	The complete amino acid sequence of human prostaglandin endoperoxide synthase (EC 1.14.99.1, cyclooxygenase) was deduced by cloning and sequence analysis of human genomic DNA coding for the enzyme. The isolated clones covered approximately 40 kilobase pairs of human gene and the protein coding region of the enzyme was distributed into eleven exons, which encoded 599 amino acid residues with a calculated molecular weight of 68,548. Human prostaglandin endoperoxide synthase exhibited 91 % amino acid identity with the sheep enzyme.
ISSN:	0006-291X 1090-2104
DOI:	10.1016/S0006-291X(89)80049-X