Volatilization of High Molecular Weight DNA by Pulsed Laser Ablation of Frozen Aqueous Solutions

DNA has been volatilized by pulsed laser ablation of a thin film of a frozen aqueous DNA solution. The target film was irradiated in vacuum by a pulsed laser at power densities sufficient to ablate the ice matrix. The expanding ablated water vapor entrained embedded DNA molecules and expelled them i...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 1989-12, Vol.246 (4937), p.1585-1587
Hauptverfasser:	Nelson, Randall W., Rainbow, Matthew J., Lohr, Dennis E., Williams, Peter
Format:	Artikel
Sprache:	eng
Schlagworte:	Ablation (Vaporization technology) Analytical, structural and metabolic biochemistry Autoradiography Biological and medical sciences Chemistry Density Desorption DNA Dna, deoxyribonucleoproteins Freezing Fundamental and applied biological sciences. Psychology Ice Laser power Lasers Molecular Weight Molecules Nucleic acids Phosphorus Radioisotopes Plasmids Pulsed lasers Solutions Spallation (Nuclear physics) Thin films Vaporizing Volatilization Volatilization (Physics)
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Beschreibung
Zusammenfassung:	DNA has been volatilized by pulsed laser ablation of a thin film of a frozen aqueous DNA solution. The target film was irradiated in vacuum by a pulsed laser at power densities sufficient to ablate the ice matrix. The expanding ablated water vapor entrained embedded DNA molecules and expelled them into the gas phase. Ejection of DNA molecules as large as 410 kilodaltons was verified by collection of the ablation products and subsequent mass analysis by polyacrylamide gel electrophoresis with autoradiographic detection.
ISSN:	0036-8075 1095-9203
DOI:	10.1126/science.2595370