Immobilization stress increases mRNA levels of interleukin-1 receptor antagonist in various rat brain regions

1. Interleukin-1 receptor antagonist (IL-1Ra), as well as the interleukin-1 beta (IL-1 beta) gene response to immobilization stress (IMS), was examined in the rat brain. The reverse transcription-polymerase chain reaction was employed to determine mRNA levels. 3. IL-1 beta and IL-1Ra mRNA levels pea...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Cellular and molecular neurobiology 1997-10, Vol.17 (5), p.557-562
Hauptverfasser:	Suzuki, E, Shintani, F, Kanba, S, Asai, M, Nakaki, T
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Brain - physiopathology Brain Chemistry - physiology Gene Expression - physiology Interleukin 1 Receptor Antagonist Protein Male Polymerase Chain Reaction Rats Rats, Sprague-Dawley Receptors, Interleukin-1 - antagonists & inhibitors Receptors, Interleukin-1 - physiology Restraint, Physical RNA, Messenger - metabolism Sialoglycoproteins - genetics Signal Transduction - physiology Stress, Physiological - physiopathology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	1. Interleukin-1 receptor antagonist (IL-1Ra), as well as the interleukin-1 beta (IL-1 beta) gene response to immobilization stress (IMS), was examined in the rat brain. The reverse transcription-polymerase chain reaction was employed to determine mRNA levels. 3. IL-1 beta and IL-1Ra mRNA levels peaked at approximately 0.5 and 2-4 hr, respectively. The maximum mRNA levels of IL-1 beta were 15-fold higher than pre-IMS levels, whereas those of IL-1Ra were 250-fold higher in the hypothalamus. 3. After the biosynthesis of IL-1 beta has peaked, IL-1Ra may contribute to attenuation of the IL-1 activity which has been enhanced by IMS.
ISSN:	0272-4340
DOI:	10.1023/a:1026319107528