Identification and characterization of a Treponema pallidum subsp. pallidum gene encoding a DNA adenine methyltransferase

Identification and characterization of a Treponema pallidum subsp. pallidum gene encoding a DNA adenine methyltransferase

The nucleotide sequence of a DNA adenine methyltransferase gene ( dam) from Treponema pallidum has been determined. Southern blot analysis of T. pallidum chromosomal DNA indicated that this gene is present as a single copy. The dam gene encodes a 303 amino acid protein whose deduced sequence has sig...

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Veröffentlicht in:FEMS microbiology letters 1997-10, Vol.155 (1), p.115-119
Hauptverfasser: Stamm, Lola V, Greene, Shermalyn R, Barnes, Natalie Y, Bergen, Heather L, Hardham, John M
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acid Sequence
Base Sequence
Conserved Sequence
dam
DNA adenine methyltransferase
DNA methylation
DNA, Bacterial - genetics
Escherichia coli - enzymology
Escherichia coli - genetics
Genes, Bacterial
Molecular Sequence Data
Plasmids - genetics
Sequence Homology, Amino Acid
Site-Specific DNA-Methyltransferase (Adenine-Specific) - genetics
Species Specificity
Streptococcus pneumoniae - enzymology
Streptococcus pneumoniae - genetics
Syphilis
Treponema pallidum
Treponema pallidum - enzymology
Treponema pallidum - genetics
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Zusammenfassung:The nucleotide sequence of a DNA adenine methyltransferase gene ( dam) from Treponema pallidum has been determined. Southern blot analysis of T. pallidum chromosomal DNA indicated that this gene is present as a single copy. The dam gene encodes a 303 amino acid protein whose deduced sequence has significant homology with DNA ( N 6-adenine) methyltransferases. T. pallidum Dam can be assigned to group α DNA amino methyltransferases based on the order of nine conserved motifs that are present in the protein. Digests of T. pallidum chromosomal DNA performed with isoschizomer restriction endonucleases ( Sau3AI, DpnI, and MboI) confirmed the presence of methylated adenine residues in GATC sequences (Dam + phenotype).
ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-1097(97)00375-3