Measurement of low-density lipoprotein particle size by high-performance gel-filtration chromatography

We describe a new technique for measuring LDL size by high-performance gel-filtration chromatography (HPGC). LDL was subjected to chromatography, and the column effluent was monitored at 280 nm. The retention time of the LDL peak was used to calculate the LDL diameter. We compared the HPGC method wi...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Clinical chemistry (Baltimore, Md.) Md.), 1997-10, Vol.43 (10), p.1904-1912
Hauptverfasser:	Scheffer, Peter G, Bakker, Stephan J. L, Heine, Robert J, Teerlink, Tom
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Chromatography, Gel - methods Chromatography, High Pressure Liquid - methods Diabetes Mellitus, Type 2 - blood Electrophoresis, Polyacrylamide Gel Female Humans Investigative techniques, diagnostic techniques (general aspects) Lipoproteins, LDL - blood Lipoproteins, LDL - chemistry Male Medical sciences Miscellaneous. Technology Particle Size Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Reproducibility of Results
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	We describe a new technique for measuring LDL size by high-performance gel-filtration chromatography (HPGC). LDL was subjected to chromatography, and the column effluent was monitored at 280 nm. The retention time of the LDL peak was used to calculate the LDL diameter. We compared the HPGC method with gradient gel electrophoresis (GGE) on 2-10% nondenaturing polyacrylamide gels. In a group of 60 non-insulin-dependent diabetes mellitus patients, LDL size as measured by HPGC and GGE was highly correlated (r = 0.88, P
ISSN:	0009-9147 1530-8561
DOI:	10.1093/clinchem/43.10.1904