The Action of 4-Hydroxynonenal on Heat Shock Gene Expression in Cultured Hepatoma Cells

Hep G2 cells do not synthesize heat-shock proteins when incubated with ADP-iron, under conditions that can trigger lipoperoxidation. However, exposure of these cells to added 4-hydroxynonenal (HNE), one of the main products of lipoperoxidation, induces the synthesis of hsp70, the most conserved amon...

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Veröffentlicht in:	Free radical research 1989, Vol.7 (3-6), p.189-194
Hauptverfasser:	Cajone, F., Bernelli-Zazzera, A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Diphosphate - pharmacology Aldehydes - pharmacology Animals Blotting, Northern Carcinoma, Hepatocellular - genetics Carcinoma, Hepatocellular - metabolism DNA Probes Electrophoresis Gene Expression - drug effects heat-shock heat-shock genes heat-shock proteins Heat-Shock Proteins - biosynthesis Heat-Shock Proteins - genetics Humans Immunoblotting Iron Chelating Agents - pharmacology Lipid peroxidation Liver Neoplasms Liver Neoplasms, Experimental - genetics Liver Neoplasms, Experimental - metabolism Neoplasm Proteins - biosynthesis Nucleic Acid Hybridization Rats RNA, Messenger - genetics RNA, Neoplasm - genetics Tumor Cells, Cultured
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Zusammenfassung:	Hep G2 cells do not synthesize heat-shock proteins when incubated with ADP-iron, under conditions that can trigger lipoperoxidation. However, exposure of these cells to added 4-hydroxynonenal (HNE), one of the main products of lipoperoxidation, induces the synthesis of hsp70, the most conserved among heat-shock proteins. HNE acts mainly on transcription: in Hep G2 cells the increase in the steady-state level of hsp70 mRNA is detectable by two different hybridization techniques.
ISSN:	1071-5762 8755-0199 1029-2470
DOI:	10.3109/10715768909087941