Cytotoxic flavonoids from Vitex agnus-castus

Four new flavonoids, luteolin 6- C-(4″-methyl-6″- O- trans-caffeoyl glucoside) ( 1), luteolin 6- C-(6″- O- trans-caffeoyl glucoside) ( 2), luteolin 6- C-(2″- O- trans-caffeoyl glucoside) ( 3), and luteolin 7- O-(6″- p-benzoyl glucoside), were isolated and the structures elucidated by spectroscopic means. Four new flavonoids, luteolin 6- C-(4″-methyl-6″- O- trans-caffeoylglucoside), luteolin 6- C-(6″- O- trans-caffeoylglucoside), luteolin 6- C-(2″- O- trans-caffeoylglucoside), and luteolin 7- O-(6″- p-benzoylglucoside), together with four known ones 5, 4′-dihydroxy-3,6,7,3′-tetramethoxyflavone, luteolin, artemetin and isorhamnetin, were isolated from the root bark of Vitex agnus-castus. The structures were elucidated by spectroscopic means.