Quantitative determination of various hopanoids in microorganisms

A rapid and sensitive method for determination of various hopanoids in microorganisms is described. Tetrahydroxybacteriohopane (THBH), THBH-ether and -glycoside were acetylated with acetanhydride/pyridine and were separated on a C18 reversed-phase HPLC column with a gradient of acetonitrile in metha...

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Veröffentlicht in:	Analytical biochemistry 1989-08, Vol.181 (1), p.120-124
Hauptverfasser:	SCHULENBERG-SHELL, H, NEUSS, B, SAHM, H
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria - analysis Bacteriology Biological and medical sciences Chromatography, Gas Chromatography, High Pressure Liquid Fundamental and applied biological sciences. Psychology Glycosides - isolation & purification Microbiology Morphology, structure, chemical composition Reference Standards Spectrophotometry, Ultraviolet Triterpenes - isolation & purification
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Zusammenfassung:	A rapid and sensitive method for determination of various hopanoids in microorganisms is described. Tetrahydroxybacteriohopane (THBH), THBH-ether and -glycoside were acetylated with acetanhydride/pyridine and were separated on a C18 reversed-phase HPLC column with a gradient of acetonitrile in methanol and subsequent monitoring of the eluant at 206 nm. Quantification is based on peak area calculation by using purified bacteriohopanoids as external standards. A linear response is found from 10 to 20 micrograms bacteriohopanetetrol and for up to 200 micrograms ether and glycoside of THBH. Diplopterol and diploptene were determined by GLC after silylation with BSTFA/TMSCl in pyridine; the detector response is linear from 0.1 to 2 micrograms.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(89)90403-X