Detrimental effect of hydrosalpinx fluid on the development and blastulation of mouse embryos in vitro

Objective: To study the effects of human hydrosalpinx fluid on mouse embryo blastulation rate. Design: Comparison of mouse embryo blastulation rate in media containing increasing concentrations of hydrosalpinx fluid. Setting: Tertiary care center. Patient(s): Women undergoing laparoscopic evaluation or treatment for infertility noted to have hydrosalpinx or paratubal cyst. Intervention(s): Exposure of mouse embryos to hydrosalpinx or paratubal cyst fluid collected during laparoscopy. Main Outcome Measure(s): Blastulation rate of mouse embryos. Result(s): Culture of mouse embryos at 0% (controls), 0.3%, 0.6%, and 0.9% hydrosalpinx fluid concentrations demonstrated significantly lower blastulation rate at each level compared with the controls. Conclusion(s): Hydrosalpinx fluid is highly embryotoxic.