By-products in the analysis of β-muricholic acid in biological samples as methyl ester triacetate

By-products were formed on analysis of β-muricholic acid (3α,6β, 7β-trihydroxy-5β-cholan-24-oic acid) in biological samples by a method involving acid-catalyzed solvolysis of sulfate esters in acetone-methanol, followed by perchloric acid-catalyzed acetylation with acetic anhydride-acetic acid. Thes...

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Veröffentlicht in:	Steroids 1989-06, Vol.53 (6), p.677-693
Hauptverfasser:	Janssen, Gerard, Toppet, Suzanne, Compernolle, Frans, Parmentier, Guido
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylation Analytical, structural and metabolic biochemistry Bile Acids and Salts - analysis Biological and medical sciences Chemical Phenomena Chemistry Cholic Acids - analysis Chromatography, Gas Deamination Enzymes - metabolism Esterification Fundamental and applied biological sciences. Psychology Magnetic Resonance Imaging Mass Spectrometry Molecular Structure Other biological molecules Terpenes, steroids. Hormones
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Beschreibung
Zusammenfassung:	By-products were formed on analysis of β-muricholic acid (3α,6β, 7β-trihydroxy-5β-cholan-24-oic acid) in biological samples by a method involving acid-catalyzed solvolysis of sulfate esters in acetone-methanol, followed by perchloric acid-catalyzed acetylation with acetic anhydride-acetic acid. These products have been identified by mass spectrometry and nuclear magnetic resonance as methyl 3-0,6-0-diacetyl-7-0-(1-methyl-3-oxo-1-butenyl)- and methyl 3-0,7-0-diacetyl-6-0-(1-methyl-3-oxo-1-butenyl)-β-muricholate, methyl 3-0,6-0-diacetyl- and methyl 3-0, 7-0-diacetyl-β-muricholate, and a methyl diacetoxy-cholen-24-oate.
ISSN:	0039-128X 1878-5867
DOI:	10.1016/0039-128X(89)90060-3