γ-Aminobutyric acid elevates cytosolic Ca in bovine chromaffin cells

Measurements of the cytosolic Ca concentration ([Ca] i) with the Ca-sensitive dye, fura-2, showed that in intact, but not in voltage-clamped, bovine chromaffin cells γ-aminobutyric acid (GABA, 10 μM) elicited a transient increase in [Ca] i. The Ca transient of intact cells was inhibited by bicuculli...

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Veröffentlicht in:	Neuroscience letters 1989-09, Vol.104 (1), p.83-87
1. Verfasser:	Doroshenko, P.A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adrenal Medulla - metabolism Animals Bicuculline Bicuculline - pharmacology Bovine adrenal medulla Calcium - metabolism Calcium channel Calcium Channels - metabolism Cattle Chloride Channels Chlorides Chromaffin cell Chromaffin System - drug effects Chromaffin System - metabolism Cobalt - pharmacology Cytosol - metabolism Cytosolic Ca Fura-2 gamma-Aminobutyric Acid - pharmacology Ion Channel Gating Membrane Potentials Membrane Proteins - physiology γ-Aminobutyric acid
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Zusammenfassung:	Measurements of the cytosolic Ca concentration ([Ca] i) with the Ca-sensitive dye, fura-2, showed that in intact, but not in voltage-clamped, bovine chromaffin cells γ-aminobutyric acid (GABA, 10 μM) elicited a transient increase in [Ca] i. The Ca transient of intact cells was inhibited by bicuculline (20 μM), by removal of extracellular Ca or by treatment with the Ca channel blocker cobalt (2.5 mM), and enhanced by lowering the extracellular Cl. We conclude, that GABA elevates [Ca] i by inducing a GABA A-receptor-linked Cl current which depolarizes the cell membrane sufficiently to activate potential-operated Ca channels and cause Ca entry into the cell.
ISSN:	0304-3940 1872-7972
DOI:	10.1016/0304-3940(89)90333-9